Platelets upon activation change their shape, aggregate and secrete alpha and dense granule contents among which ADP acts as a feedback activator. Different Protein Kinase C (PKC) isoforms have specific non-redundant roles in mediating platelet responses including secretion and thrombus formation. Murine platelets lacking specific PKC isoforms have been used to evaluate the isoform specific functions. Novel PKC isoform δ has been shown to play an important role in some pathological processes. Lack of specific inhibitors for PKCδ has restricted analysis of its role in various cells. The current study was carried out to evaluate a novel small molecule PKCδ inhibitor, CGX1037 in platelets. Platelet aggregation, dense granule secretion and western blotting experiments were performed to evaluate CGX1037. In human platelets, CGX1037 inhibited PAR4-mediated phosphorylation on PKD2, a PKCδ-specific substrate. Pre-treatment of human or murine platelets with CGX1037 inhibited PAR4-mediated dense granule secretion whereas it potentiated GPVI-mediated dense granule secretion similar to the responses observed in murine platelets lacking PKCδ· Furthermore, pre-treatment of platelets from PKCδ(-/-) mice with CGX1037 had no significant additive effect on platelet responses suggesting the specificity of CGX1037. Hence, we show that CGX1037 is a selective small molecule inhibitor of PKCδ in platelets.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101062 | PMC |
| http://dx.doi.org/10.3109/09537104.2013.868877 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of transglutaminase on the interaction of protein and rice starch.
Int J Biol Macromol
December 2024
National Engineering Research Center of Rice and Byproduct Deep Processing, Central South University of Forestry & Technology, Changsha 410004, PR China.
The present study aims to investigate the effects of endogenous protein (rice protein, RP) and exogenous proteins (corn protein, CP, and wheat protein, WP) on the physicochemical properties of rice starch under the action of transglutaminase (TG). The findings indicate that, the interactions between exogenous proteins with rice starch are relatively weak. However, with the catalysis of TG, both endogenous and exogenous proteins tightly encapsulate rice starch granules, forming a dense microporous network structure.
View Article and Find Full Text PDFBioestratinomic Classification and Genesis of Shell Beds (Coquinas) from the Santa Marta Formation (Upper Cretaceous) of James Ross Island, Antarctica.
An Acad Bras Cienc
December 2024
Universidade Federal do Rio de Janeiro, Departamento de Geologia e Paleontologia, Laboratório de Sistemática e Tafonomia de Vertebrados Fósseis (LAPUG), Museu Nacional, Campus de Pesquisa e Ensino, Avenida Bartolomeu de Gusmão, 875, São Cristóvão, 20941-160 Rio de Janeiro, RJ, Brazil.
Shell beds, or coquinas, have a complex origin, limiting their utility in paleoecology. However, such accumulations can serve as crucial paleoenvironmental indicators, since their bioestratinomic and diagenetic properties explain the physical-chemical and biological processes of their formation, as well as the ancient environments linked to their development. In 2016, the PALEOANTAR Project sampled coquinas from the James Ross Island (Antarctic Peninsula) in Passo São José (PSJ - San José Way) and Muro do Castelo (MDC - Castle Wall), two new localities with outcrops of the lower Lachman Crags Member, Santa Marta Formation, Marambio Group, Cretaceous of Larsen Basin.
View Article and Find Full Text PDFArticle Synopsis
- The obligate intracellular parasite replicates within a compartment called the parasitophorous vacuole (PV) and utilizes a protein ingestion pathway to take in nutrients from the host cell's cytosol, initiated by the protein GRA14.
- A genome-wide CRISPR screen revealed that mutants lacking components of this ingestion pathway (GRA14, CPL, or CRT) are forced to rely more on alternative metabolic pathways to survive, such as pyrimidine and fatty acid biosynthesis.
- Analysis showed that these ingestion-deficient mutants had lower levels of key nutrients and growth defects when amino acids were scarce, indicating that the ingestion pathway plays a crucial role in nutrient acquisition during resource-limited conditions.
Article Synopsis
- - Three new species of brachyuran crabs have been identified in Tamil Nadu, India, including the first epialtid species from this region, Acanthophrys keeae n. sp., which has unique physical features that distinguish it from related species in Australia.
- - The second species, Latopilumnus ajmali n. sp., was discovered in a sponge and is characterized by its densely hairy body and smooth dorsal surface with poorly defined regions.
- - The third species, Vellumnus intonsus n. sp., found in coral rubble, shares similarities with two other species but can be identified by specific features such as a deep cleft separating lobes on its front and distinctive shapes of
Quantitative and qualitative assessment of a pituitary neuroendocrine tumor's T2-signal intensity in acromegaly - a call for unification.
Front Endocrinol (Lausanne)
December 2024
Chair and Department of Endocrinology, Jagiellonian University Medical College, Krakow, Poland.
Introduction: The T2-signal intensity (SI) of somatotroph pituitary neuroendocrine tumors (sPitNET) is associated with treatment response and granulation pattern. Our aim was to evaluate SI assessment methods and their clinical implications, including responsiveness to preoperative first-generation somatostatin analogs (SSA).
Methods: This single-center, observational study included unselected, consecutive patients with newly diagnosed acromegaly.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!