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Localization of beta-hydroxysteroid dehydrogenase in Anguilla anguilla testis. | LitMetric

AI Article Synopsis

  • The histochemical reaction for 3 beta-hydroxysteroid dehydrogenase was used on glutaraldehyde fixed eel testis, which was later processed for electron microscopy.
  • The reaction showed positive results solely in the smooth endoplasmic reticulum and the intermembrane space of mitochondria in the Leydig cells of hCG treated silver eels.
  • The observed positive reaction is linked to the enlargement of Leydig cells and a rise in their smooth endoplasmic reticulum.

Article Abstract

The histochemical reaction for 3 beta-hydroxysteroid dehydrogenase was applied to glutaraldehyde fixed eel testis and successively processed for transmission electron microscopy. The reaction product was found only on the smooth endoplasmic reticulum and in the intermembrane space of mitochondria of the Leydig cells of hCG treated silver eels. The positive cytochemical reaction appears to be coupled with an enlargement of the Leydig cells and an increase of their smooth endoplasmic reticulum.

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