A loop-mediated isothermal amplification (LAMP) system, the illumigene Mycoplasma DNA amplification assay (Meridian Bioscience, Inc., Cincinnati, OH) was evaluated to determine its analytical sensitivity, specificity, and clinical application in comparison to historic culture in a collection of archived respiratory specimens. The illumigene limit of detection was ≤88 CFU/reaction for 10 Mycoplasma pneumoniae reference strains. This assay correctly identified 36 M. pneumoniae reference strains and clinical isolates from various geographic origins, including both of the main subtypes. No cross-reactions were detected with other mycoplasmas, ureaplasmas, other bacterial species, viruses, yeasts, or human DNA. Among 214 respiratory specimens previously cultured for M. pneumoniae, when real-time PCR with bidirectional sequencing of the PCR products was used to resolve discrepancies, the sensitivity was 22 of 22 (100%) and the specificity was 190 of 192 (99%). This commercial LAMP assay is a useful rapid method for detecting M. pneumoniae in clinical specimens. Additional prospective clinical trials with direct comparison to culture and PCR are warranted.
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http://dx.doi.org/10.1128/JCM.02913-13 | DOI Listing |
Acta Clin Belg
November 2018
a Belgian Reference Centre for Respiratory Pathogens , University Hospital Antwerp, Belgium.
J Clin Microbiol
July 2018
Children's Mercy Hospital and Clinics, Kansas City, Missouri, USA
is a common cause of community-acquired pneumonia. The Mycoplasma Direct (iMD) DNA amplification assay is a qualitative test utilizing loop-mediated isothermal amplification (LAMP) technology for the direct detection of DNA in respiratory specimens. The iMD assay does not require the preextraction of nucleic acids from specimens, which is a prerequisite step for the previously approved Mycoplasma (iM) assay.
View Article and Find Full Text PDFJ Clin Microbiol
April 2014
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
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