Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli.

World J Microbiol Biotechnol

Departament de Microbiologia, Facultat de Ciències Biològiques, Universitat de València, Calle Dr. Moliner 50, 46100, Valencia, Spain.

Published: March 1992

A promoter probe library from the dermatophyte fungusTrichophyton mentagrophytes has been constructed in the pVB32 plasmid vector. Using this library, a set ofT. mentagrophytes DNA sequences with promoter activity inEscherichia coli has been cloned. The size and the resistance phenotype conferred by these DNA fragments varied. Southern blot analysis confirmed that they were derived fromT. mentagrophytes genomic DNA.

Download full-text PDF

Source
http://dx.doi.org/10.1007/BF01195847DOI Listing

Publication Analysis

Top Keywords

mentagrophytes dna
8
dna sequences
8
sequences promoter
8
promoter activity
8
activity inescherichia
8
inescherichia coli
8
molecular cloning
4
cloning oftrichophyton
4
mentagrophytes
4
oftrichophyton mentagrophytes
4

Similar Publications

Epidemiological studies combining taxonomic and clinical data have been limited globally, particularly Guiyang, the most under-developed economic provincial capital city in southwestern China. A retrospective analysis was performed of dermatophyte epidemiology involving all culture-positive cases received between May 2017 and May 2023 at the Affiliated Hospital of Guizhou Medical University. Phylogenetic analysis was conducted on 391 dermatophyte isolates collected from patients using the rDNA internal transcribed spacer sequences.

View Article and Find Full Text PDF

Background: Trichophyton indotineae, formerly described as T. mentagrophytes rDNA-ITS genotype VIII, has recently been identified as a novel species within the T. mentagrophytes complex.

View Article and Find Full Text PDF

Introduction Dermatophytes are the most common cause of superficial fungal infection. They are mostly diagnosed using phenotypic methods, but recently the molecular methods seem to be gaining ground. The objective of the present study was to compare the phenotypic and genotypic methods of identification of dermatophytes and understand the feasibility of using molecular methods for routine diagnosis of dermatophytosis.

View Article and Find Full Text PDF

Objectives: Tinea capitis remains a common fungal infection in children worldwide. Species identification is critical for determining the source of infection and reducing transmission. In conventional methods, macro- and microscopic analysis is time-consuming and results in slow fungal growth or low specificity.

View Article and Find Full Text PDF

Molecular epidemiology of dermatophytosis in Golestan, Iran: A cross-sectional study.

Microb Pathog

February 2025

Invasive Fungi Research Center, Communicable Diseases Institute/Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address:

Keratinophilic fungi, or dermatophytes, are recognized as the predominant fungal agents responsible for superficial skin diseases globally. The identification of species of dermatophytes is crucial for both therapeutic and epidemiological considerations. The primary objective of the present study was to investigate the epidemiology of dermatophytosis among patients who sought medical attention at the medical mycology laboratory in Golestan province.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!