A promoter probe library from the dermatophyte fungusTrichophyton mentagrophytes has been constructed in the pVB32 plasmid vector. Using this library, a set ofT. mentagrophytes DNA sequences with promoter activity inEscherichia coli has been cloned. The size and the resistance phenotype conferred by these DNA fragments varied. Southern blot analysis confirmed that they were derived fromT. mentagrophytes genomic DNA.
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http://dx.doi.org/10.1007/BF01195847 | DOI Listing |
Mycopathologia
January 2025
Department of Dermatology, The Affiliated Hospital of Guizhou Medical University, Beijing Road 4, Yunyan District, Guiyang, China.
Epidemiological studies combining taxonomic and clinical data have been limited globally, particularly Guiyang, the most under-developed economic provincial capital city in southwestern China. A retrospective analysis was performed of dermatophyte epidemiology involving all culture-positive cases received between May 2017 and May 2023 at the Affiliated Hospital of Guizhou Medical University. Phylogenetic analysis was conducted on 391 dermatophyte isolates collected from patients using the rDNA internal transcribed spacer sequences.
View Article and Find Full Text PDFMycoses
January 2025
Mycology Reference Laboratory, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Trichophyton indotineae, formerly described as T. mentagrophytes rDNA-ITS genotype VIII, has recently been identified as a novel species within the T. mentagrophytes complex.
View Article and Find Full Text PDFCureus
November 2024
Microbiology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, IND.
Introduction Dermatophytes are the most common cause of superficial fungal infection. They are mostly diagnosed using phenotypic methods, but recently the molecular methods seem to be gaining ground. The objective of the present study was to compare the phenotypic and genotypic methods of identification of dermatophytes and understand the feasibility of using molecular methods for routine diagnosis of dermatophytosis.
View Article and Find Full Text PDFMycoses
January 2025
Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China.
Objectives: Tinea capitis remains a common fungal infection in children worldwide. Species identification is critical for determining the source of infection and reducing transmission. In conventional methods, macro- and microscopic analysis is time-consuming and results in slow fungal growth or low specificity.
View Article and Find Full Text PDFMicrob Pathog
February 2025
Invasive Fungi Research Center, Communicable Diseases Institute/Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address:
Keratinophilic fungi, or dermatophytes, are recognized as the predominant fungal agents responsible for superficial skin diseases globally. The identification of species of dermatophytes is crucial for both therapeutic and epidemiological considerations. The primary objective of the present study was to investigate the epidemiology of dermatophytosis among patients who sought medical attention at the medical mycology laboratory in Golestan province.
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