Measurements of phytochrome by Δ (ΔA725-815 nm) were completely suppressed at chlorophyll concentrations of the order of 20-40 μg g(-1) f.wt. in vivo and 37 μg cm(-3) in vitro, and the readings were reduced by 50% at only 12 μg cm(-3) in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.
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