AI Article Synopsis
- Researchers identified two potent bacterial isolates, Bacillus laterosporus and Flavobacterium sp., from gelatin factory effluents in Jabalpur, India, which showed strong protease activity.
- Both bacterial enzymes performed best at a pH of 8 and a temperature of 60°C, peaking in activity after 90 minutes of incubation.
- Bacillus laterosporus enzyme activity was negatively affected by certain metal ions (Fe, Mg, Mn, Zn) but positively influenced by Ba and Ca, while Flavobacterium sp. had a similar response with some variations in ion sensitivities.
Article Abstract
Forty bacterial isolates from the effluents of a gelatin factory (Jabalpur, India) were screened for protease activity and the two most potent producers were identified as Bacillus laterosporus and a Flavobacterium sp. The enzymes of both isolates were optimal at pH 8 and 60°C, with maximum activity after 90 min. The enzyme activity of B. laterosporus was suppressed by Fe(2+), Mg(2+), Mn(2+) and Zn(2+) ions but was enhanced by Ba(2+) and Ca(2+). That of Flavobacterium sp. was suppressed by Mg(2+) and Mn(2+) ions but enhanced by Ba(2+), Ca(2+) and Fe(2+). The enzyme activity of the former was strongly inhibited by KCN, whereas that of the latter was only slightly inhibited by 8-hydroxyquinoline.
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