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Short communication: Protoplast formation from the thermophilic fungus Malbranchea sulfurea, using the thermostable chitinase and laminarinase of Paecilomyces varioti. | LitMetric

AI Article Synopsis

  • Two enzymes, chitinase and laminarinase, from the thermophilic fungus Paecilomyces varioti were used to extract protoplasts from another thermophilic fungus, Malbranchea sulfurea.
  • The success rate of protoplast regeneration was found to be 35%.
  • This regeneration rate significantly outperformed results obtained through the use of commercial lytic enzymes.

Article Abstract

Two thermostable enzymes produced by the thermophilic fungus Paecilomyces varioti, a chitinase and laminarinase, were used to isolate protoplasts of a thermophilic fungus, Malbranchea sulfurea. The frequency of protoplast regeneration observed (35%) was considerably higher than that obtained using commercial lytic enzymes.

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Source
http://dx.doi.org/10.1007/BF00327811DOI Listing

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