AI Article Synopsis
Article Abstract
This paper describes the development of an immunosensor for detection of anti-hemagglutinin antibodies. Its preparation consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibody, (iv) immobilization of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA. The interactions between two variants of recombinant HA (short and long) from highly pathogenic avian influenza virus H5N1 and the anti-H5 HA monoclonal antibody (Mab 6-9-1) have been explored with electrochemical impedance spectroscopy (EIS). The impedimetric immunosensor displayed a very good detection limit (LOD) of 2.1 pg/mL, the quantification limit (LOQ) of 6.3 pg/mL and a dynamic range from 4 pg/mL to 20 pg/mL. In addition, this analytical device was applied for detection of antibodies against His6-H5 HA in serum of vaccinated hen using serial 10-fold dilutions of serum. The immunosensor proposed was able to detect antibody in hen serum diluted up to 7 × 10(7)-fold. The sensitivity of immunosensor was about four orders of magnitude much better than ELISA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2013.12.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sandwich-type electrochemical immunosensor based on Au NPs/3D hierarchical porous carbon network and Au NPs/CuS nanocages for the detection of alpha-fetoprotein.
Colloids Surf B Biointerfaces
December 2024
The Affiliated Guangdong Second Provincial General Hospital of Jinan University, PR China. Electronic address:
Alpha-fetoprotein (AFP), serves as a reliable and vital biomarker for precise diagnosis and effective monitoring of hepatocellular carcinoma, requires precise detection. Herein, a sandwich-structured electrochemical immunosensor was crafted, employing three-dimensional layered porous carbon modified with gold nanoparticles (Au NPs) as the substrate and Au NPs/CuS as the labeling compound for accurate and sensitive detection of AFP. Due to the effective coordination between the 3D carbon network, Au NPs, and hollow CuS nanocubes, the sandwich-structured electrochemical immunosensor was able to produce three distinct response signals via various detection techniques, demonstrating a broad linear range (0.
View Article and Find Full Text PDFEmerging bioengineering breakthroughs in precision diagnosis and therapy for endometriosis and adenomyosis.
J Mater Chem B
December 2024
Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.
Endometriosis and adenomyosis are debilitating gynecological conditions that severely affect the quality of life of women. Traditional diagnostic and treatment methods, including laparoscopic surgery and hormonal therapy, face significant limitations such as incomplete lesion detection, high recurrence rates, and adverse side effects. Emerging bioengineering technologies offer promising solutions for precise diagnosis and therapy of these diseases.
View Article and Find Full Text PDFElectrochemiluminescence immunoassay of cTnI with ruthenium-based metal covalent organic framework and dual DNAzymes cascade amplification strategy.
Chemistry
December 2024
Southwest University, school of chemistry and chemical enjineering, 2 Tiansheng Road, Beibei District, Chongqing, 400700, Chongqing, CHINA.
Currently, metal-organic frameworks (MOFs) with tunability and covalent organic frameworks (COFs) with high stability are promising nanomaterials for electrochemiluminescence (ECL), while Ru-based metal covalent organic frameworks (Ru-MCOFs) have rarely been reported. Herein, an ECL immunosensor based on a strong ECL-emitting Ru-MCOF was proposed for the sensitive detection of the cardiac troponin-I (cTnI). Imine-linked Ru-MCOF was prepared as an ECL emitter via solvothermal method using tris(4,4' -diamino-2,2' -bipyridine)ruthenium(II) (Ru(dbpy)32+) as a precursor.
View Article and Find Full Text PDFManganese porphyrin wrapped DNA dendrimer as a universal chemiluminescence tag for ultrasensitive and multiplex assay of mycotoxins.
Talanta
December 2024
State Key Laboratory of Natural Medicines, China Pharmaceutical University, China Pharmaceutical University, Nanjing, 211198, PR China. Electronic address:
A manganese porphyrin wrapped DNA dendrimer (Mn-DD) was developed through enzyme-free DNA self-assembly and simple and mild groove binding of porphyrin. The Mn-DD not only possessed plenty of manganese porphyrin to amplify the chemiluminescence (CL) signal, but also can be modified with diverse groups via DNA hybridization. Combined with an immunosensor array, Mn-DD can be utilized for CL immunoassay of multiple mycotoxins as a universal tag.
View Article and Find Full Text PDFMXene-rGO nanocomposite based electrochemical immunosensor for detection of endosulfan - An organochlorine pesticide.
Chemosphere
December 2024
BRIC-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India; BRIC- Regional Centre for Biotechnology (RCB), Faridabad, 121001, Haryana, India. Electronic address:
Endosulfan (Ed), a widely used organochlorine pesticide, is classified as a persistent organic pollutant (POP). Its long half-life, resistance to degradation, and bioaccumulation in the food chain contaminates soil, water, and air. Such widespread environmental damage triggers monitoring its levels for ensuring compliance with safety regulations and protecting public health.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!