AI Article Synopsis
- Researchers developed a one-pot method using three enzymes to efficiently produce ADP-d-glycero-β-d-manno-heptose (ADP-d, d-heptose).
- The process involves chemically synthesized d, d-heptose-7-phosphate and the biosynthetic enzymes HldE and GmhB.
- Additionally, it was found that the enzyme HldE has very limited substrate specificity, only acting on certain modified heptose-7-phosphate analogs.
Article Abstract
An efficient one-pot three enzymes strategy for chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose (ADP-d, d-heptose) was reported using chemically synthesized d, d-heptose-7-phosphate and the ADP-d, d-heptose biosynthetic enzymes HldE and GmhB. Moreover, the result of investigating substrate specificity of the kinase action of HldE revealed that HldE had highly restricted substrate specificity towards structurally modified heptose-7-phosphate analogs.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976583 | PMC |
| http://dx.doi.org/10.1016/j.bmc.2013.12.019 | DOI Listing |
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