AI Article Synopsis
- The study involved 40 finished pigs from a single herd infected with Mycobacterium avium subsp. avium, focusing on tissue samples collected during postmortem inspection.
- 107 samples were analyzed, including lymph nodes from pigs with and without visible tuberculosis lesions, revealing that half of the pigs had detectable levels of M. a. avium in their tissues.
- The qPCR method successfully quantified M. a. avium in diaphragm tissues with TB-like lesions, suggesting its potential usefulness for assessing foodborne risks.
Article Abstract
This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)-like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.
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| http://dx.doi.org/10.4315/0362-028X.JFP-13-036 | DOI Listing |
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