Objective: To explore the relationship between multi-differentiation potential of monoclonal immortalized human mesenchymal stem cells (hMSC-TERT) in vivo and their cluster of differentiation antigens (CD).
Methods: Monoclonal hMSC-TERT were isolated using limiting dilution. Direct immunofluorescence staining flow cytometry was used to detect the cluster of differentiation antigens (CD44, CD45, CD105) of these cell lines. Their adipocytic, osteogenic, neuronal differentiation potential in vitro were determined by Oil Red O staining, Von Kossa staining and immunocytochemistry for tubulin-β III antibody. Then hMSC-TERTs were transplanted subcutaneously into severe combined immunodeficiency (SCID) mice. The cell grafts were removed and analyzed by immunohistochemistry for pathologic tissue markers for multi-differentiation potential of hMSC-TERT cells in vivo.
Results: CD105+ cell was 84.68% positive in hMSC-TERT-C19 while <5% in other monoclonal cell lines. The positive rate of cytokeratin in grafts formed by hMSC-TERT-C19 cell line in SCID mice was much higher than the others (P < 0.01).
Conclusion: The positive CD105 of monoclonal hMSC-TERTs may be directly correlated with its epithelial differentiation potential.
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