Aim: To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype in vitro.
Methods: Single keratocytes were isolated by enzyme digestion for in vitro culture. Three groups were designed according to the different culture media: a bovine pituitary extract (BPE) group, a fetal bovine serum (FBS) group and the control group. The phenotypes and proliferation of cultured cells were evaluated by morphology, immunofluorescent staining and mRNA expression of CD34, Lumican, VSX1, α-SMA and proliferating cell nuclear antigen (PCNA). In the BPE group, cells underwent serial subcultivation, and their phenotypes were identified by immunofluorescent staining. To analyze the proliferation of keratocytes in different concentrations of BPE, six different concentrations were designed to ascertain the most appropriate amount.
Results: In the BPE group, the cells spread out and presented dendritic morphology, and their dendrites connected to one another to form networks. On the third passage, most cells maintained their phenotype. In the FBS group, the cells exhibited a dendritic appearance in early cultured stages, but their morphology subsequently changed into a fibroblast-like shape. The number of dendritic cells in BPE group was more than FBS and control groups. Immunofluorescent staining and real-time polymerase chain reaction (PCR) confirmed that few keratocytes underwent fibroblastic transformation in the BPE and control groups, and that proliferation was higher in the BPE group than in the control group. Although the proliferation was higher in the FBS group, many keratocytes underwent fibroblastic transformation. The analysis of cell morphology and mRNA expressions of CD34, PCNA and VSX1 in six group showed that different concentrations of BPE affected the proliferation obviously but didn't affect the keratocyte phenotype, and the concentration of 40µg/mL was the most appropriate one.
Conclusion: BPE can improve the proliferation of keratocytes and maintain their phenotype in vitro. Many keratocytes can be harvested rapidly and provide seeds for the construction of corneal stroma.
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http://dx.doi.org/10.3980/j.issn.2222-3959.2013.06.04 | DOI Listing |
Environ Sci Technol
January 2025
School of Ecology and Environmental Science, Yunnan University, Kunming 650504, China.
Safer chemical alternatives to bisphenol (BP) have been a major pursuit of modern green chemistry and toxicology. Using a chemical similarity-based approach, it is difficult to identify minor structural differences that contribute to the significant changes of toxicity. Here, we used omics and computational toxicology to identify chemical features associated with BP analogue-induced embryonic toxicity, offering valuable insights to inform the design of safer chemical alternatives.
View Article and Find Full Text PDFInt Urol Nephrol
December 2024
Department of Urology, Charité-University Hospital Berlin, Hindenburgdamm 30, 12203, Berlin, Germany.
Background: According to the European Association of Urology guidelines, the limit for monopolar, transurethral resection (M-TURP) in BPH- therapy is a volume of 80 g. However, whether larger prostates can also be resected transurethrally might also depend on the experience of the surgeon and especially the resected volume and speed of the resection. Little is known about the latter, and this paper aims to these factors.
View Article and Find Full Text PDFJ Environ Manage
January 2025
College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China; State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Shandong, Qingdao, 266580, China.
Microbiologically induced corrosion (MIC) is widespread in the oilfield industry, and new environmentally friendly materials are urgently needed to inhibit MIC with the increasing environmental requirements and microbial resistance problems. The synthesis method and cost of the materials are important factors that must be considered in the production and application. In this study, Ag/Cu bimetallic nanoparticles (BNPs) were synthesized by eco-friendly and sustainable method using waste banana peel extract (BPE) as a green reducing.
View Article and Find Full Text PDFCancers (Basel)
December 2024
Department of Medicine and Surgery, LUM University "Giuseppe Degennaro", Casamassima, 70010 Bari, Italy.
Hodgkin lymphoma (HL) treatment has dramatically improved, with high survival rates in early stages. However, long-term survivors face an increased risk of secondary cancers, particularly breast cancer (BC), which emerge as a leading cause of mortality decades after therapy. : This study explores the risk of BC and the toxic effects of radiation therapy (RT) in long-term HL survivors compared to age-matched high-risk women, including BRCA1 and BRCA2 mutation carriers.
View Article and Find Full Text PDFEur J Radiol Open
December 2024
Institute of Clinical Medicine, School of Medicine, Clinical Radiology, University of Eastern Finland, P.O. Box 1627, Kuopio Fl 70211, Finland.
Objectives: To examine the background parenchymal enhancement (BPE) levels in peri-menopausal breast MRI compared with pre- and post-menopausal breast MRI.
Methods: This study included 562 patients (55.8±12.
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