The state of "viable but non-culturable" (VBNC) is a survival strategy adopted by microorganisms when exposed to environmental stress. With the increasingly serious problem of xenobiotics pollution, enhanced microbial processes that exploit the potential of microbes to remediate polychlorinated biphenyl-contaminated environments have been developed. Microorganisms represent a significant advance with respect to the transformation and degradation of polychlorinated biphenyls in the environment. It is of great importance to study the potential function of VBNC bacteria in polluted environment. In this paper, current research status of VBNC bacteria is summarized, and resuscitation of VBNC bacteria to potentially stimulate microbial degradation of pollutants is discussed in detail. Furthermore, we put forward a novel approach to explore the potential of VBNC bacteria for polychlorinated biphenyls degradation using resuscitation promoting factor (Rpf) of Micrococcus luteus. The novel efficient method is helpful for excavating and obtaining highly desirable polychlorinated biphenyls degrading microorganisms. Moreover, the prospect of VBNC bacteria to other environmental remediation fields, such as flocculation and nitrification deodorant, is addressed.
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Environ Microbiol
January 2025
Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland.
Legionella pneumophila, the causative agent of Legionnaires' disease, employs the Icm/Dot Type IV secretion system (T4SS) to replicate in amoebae and macrophages. The opportunistic pathogen responds to stress by forming 'viable but non-culturable' (VBNC) cells, which cannot be detected by standard cultivation-based techniques. In this study, we document that L.
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Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of Russian Academy of Sciences" (FRC PSCBR RAS), 142290 Pushchino, Russia.
Phenol and its chlorinated derivatives are introduced into the environment with wastewater effluents from various industries, becoming toxic pollutants. Phenol-degrading bacteria are important objects of research; among them, representatives of the genus are often highlighted as promising. Strain 7Ba was isolated by enrichment culture.
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Meat Safety and Quality Research Unit, U.S. Department of Agriculture, U.S. Meat Animal Research Center, Clay Center, NE, United States.
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Food Safety Department, Teagasc Food Research Centre, Ashtown, Dublin, Ireland.
Controlling Listeria monocytogenes and its associated biofilms in the food industry requires various disinfection techniques, including physical, chemical, and biological treatments. Biocides, owing to their ease of use, cost-effectiveness, dissolvability in water, and efficacy against a wide range of microorganisms, are frequently selected options. Nonetheless, concerns have been raised about their efficacy in controlling L.
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December 2024
Biotechnical Faculty, Department of Food Science and Technology, University of Ljubljana, Ljubljana, Slovenia.
Campylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a survival strategy. This study presents an innovative application of NanoLuc bioluminescence to accurately monitor the development of C.
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