Main principles for carrying out of the thiobarbituric acid (TBA)-test of blood serum were characterized. These principles involved incubation of the sample at pH 1.3-1.6, extraction of the reaction products with butanol and spectrofluorimetric or spectrophotometric estimation. Content of TBA-active products in blood serum of healthy donors constituted 3.86 +/- 0.66 nmol malonic dialdehyde per ml of blood serum as shown in fluorimetric estimation by means of the procedure developed.
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