Distribution of the enzymes of template-dependent and template-independent polynucleotide syntheses (DNA-polymerase I, RNA-polymerase, polynucleotide phosphorylase) as well as those of the biosynthesis of nucleic acids precursors (nucleotide kinases, acetokinase and nucleoside deoxyribosyltransferases) during fractionation of Escherichia coli MRE-600 cell extract was studied. On the basis of the results obtained a technological scheme was developed that enabled to combine routine procedures of purification of the above mentioned enzymes.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The Passage of Chaperonins to Extracellular Locations in Requires a Functional Dot/Icm System.
Biomolecules
January 2025
Department of Microbiology-Immunology, Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
HtpB, the chaperonin of the bacterial pathogen , is found in extracellular locations, even the cytoplasm of host cells. Although chaperonins have an essential cytoplasmic function in protein folding, HtpB exits the cytoplasm to perform extracellular virulence-related functions that support 's lifestyle. The mechanism by which HtpB reaches extracellular locations is not currently understood.
View Article and Find Full Text PDFAntimicrobial Activities of Polysaccharide-Rich Extracts from the Irish Seaweed , Generated Using Green and Conventional Extraction Technologies, Against Foodborne Pathogens.
Mar Drugs
January 2025
Section of Food and Nutrition, School of Agriculture and Food Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland.
A rise in antimicrobial resistance coupled with consumer preferences towards natural preservatives has resulted in increased research towards investigating antimicrobial compounds from natural sources such as macroalgae (seaweeds), which contain antioxidant, antimicrobial, and anticancer compounds. This study investigates the antimicrobial activity of compounds produced by the Irish seaweed against and , bacterial species which are relevant for food safety. Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), ultrasound-microwave-assisted extraction (UMAE), and conventional extraction technologies (maceration) were applied to generate extracts from , followed by their preliminary chemical composition (total phenolic content, total protein content, total soluble sugars) and antimicrobial activity (with minimum inhibitory concentration determined by broth microdilution methods), examining also the molecular weight distribution (via high performance size exclusion chromatography) and oligosaccharide fraction composition (via high-performance liquid chromatography) of the polysaccharides, as they were the predominant compounds in these extracts, aiming to elucidate structure-function relationships.
View Article and Find Full Text PDFComparative Analysis of Freeze-Dried Mushroom Powders on Probiotic and Harmful Bacteria and Its Bioactive Compounds.
J Fungi (Basel)
December 2024
Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Street 138, 4032 Debrecen, Hungary.
(oyster mushroom) holds excellent promise worldwide, bringing several opportunities and augmenting the tool sets used in the biotechnology field, the food industry, and medicine. Our study explores the antimicrobial and probiotic growth stimulation benefits of freeze-dried powders (OMP-TF, oyster mushroom powder from the total fresh sample; OMP-CSR, oyster mushroom powder from the cooked solid residue; OMP-CL, oyster mushroom powder from the cooked liquid), focusing on their bioactive compounds and associated activities. Our research examined polysaccharide fractions-specifically total glucans and α- and β-glucans-alongside secondary metabolites, including polyphenols and flavonoids, from freeze-dried mushroom powders.
View Article and Find Full Text PDFIs Membrane Filtration Applicable for the Recovery of Biologically Active Substances from Spent Lavender?
Membranes (Basel)
January 2025
Department of Chemical Engineering, University of Chemical Technology and Metallurgy, 1576 Sofia, Bulgaria.
This study explored the batch membrane filtration of 40% ethanol extracts from spent lavender, containing valuable compounds like rosmarinic acid, caffeic acid, and luteolin, using a polyamide-urea thin film composite X201 membrane. Conducted at room temperature and 20 bar transmembrane pressure, the process demonstrated high efficiency, with rejection rates exceeding 98% for global antioxidant activity and 93-100% for absolute concentrations of the target components. During concentration, the permeate flux declined from 2.
View Article and Find Full Text PDFPickering emulsion stabilized by hollow Zein/SSPS nanoparticles loaded with Thymol: Formation, characterization, and application in fruit preservation.
Food Res Int
February 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:
Using Pickering emulsion (PE) as the carrier of active compounds in bio-based coatings constitutes a highly promising research domain. This study focused on creating a food-grade, biocompatible, and antibacterial PE to coat fresh fruits and vegetables, extending their shelf life. Hollow zein/soluble soybean polysaccharide nanoparticles loaded with thymol (H-ZSH/T) were produced using NaHCO as a sacrificial template to stabilize PE.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!