AI Article Synopsis
- Scientists expressed codon-optimized genes crtB and crtI from Pantoea ananatis in Yarrowia lipolytica, using the TEF1 promoter to enhance lycopene production.
- To boost isoprenoid biosynthesis, they overexpressed key genes GGS1 and HMG1 in Y. lipolytica, and also modified the strain by deleting specific lipid metabolism genes to increase lipid body size and thus lycopene production.
- As a result, the modified Y. lipolytica achieved a yield of 16 mg g(-1) (dry cell weight) of lycopene in fed-batch cultures, marking the highest yield reported for a eukaryotic host to date.
Article Abstract
The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica, GGS1 and HMG1, were overexpressed to increase the production of lycopene. All of the genes were also expressed in a Y. lipolytica strain with POX1 to POX6 and GUT2 deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene production. Lycopene is located mainly within lipid bodies, and increased lipid body formation leads to an increase in the lycopene storage capacity of Y. lipolytica. Growth-limiting conditions increase the specific lycopene content. Finally, a yield of 16 mg g(-1) (dry cell weight) was reached in fed-batch cultures, which is the highest value reported so far for a eukaryotic host.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957620 | PMC |
| http://dx.doi.org/10.1128/AEM.03167-13 | DOI Listing |
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