AI Article Synopsis
- Directional cellular migrations in response to uneven growth factor stimulation are crucial for both normal and abnormal cell functions.
- Researchers created EGF-immobilized microbeads to test their effects on fibroblast movement, finding that they triggered long-lasting and directed cell migration.
- This technique enhances the understanding of how cells respond to spatially varied stimulation of growth factors, aiding in the study of cellular signaling processes.
Article Abstract
Directional cellular migrations as a chemotactic response to spatially inhomogeneous growth factor stimulation play an important role in establishing physiological mechanisms and pathological events in cells. We developed epidermal growth factor (EGF)-immobilized microbeads by photoreaction and evaluated its local stimulatory effects on the dynamic chemotactic motility of fibroblasts. The local stimulation resulted in global activation of ERK 1/2 and directionality of cellular migration. The cellular migration by stimulation using 3-μm diameter EGF-immobilized microbeads persisted for a longer time, were involved a wider field and their number were further increased with stimulation. This effective technique allows cellular migration and biochemical analyses that will help elucidate the mechanisms involved in signal transduction by spatially inhomogeneous stimulation of the growth factor.
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| http://dx.doi.org/10.1016/j.biomaterials.2013.12.013 | DOI Listing |
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Dynamic chemotactic response of fibroblasts to local stimulation using EGF-immobilized microbeads.
Biomaterials
March 2014
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Article Synopsis
- Directional cellular migrations in response to uneven growth factor stimulation are crucial for both normal and abnormal cell functions.
- Researchers created EGF-immobilized microbeads to test their effects on fibroblast movement, finding that they triggered long-lasting and directed cell migration.
- This technique enhances the understanding of how cells respond to spatially varied stimulation of growth factors, aiding in the study of cellular signaling processes.
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