AI Article Synopsis
- The study investigates the effects of recombinant bacille Calmette-Guerin (rBCG) that expresses Der p2 on asthma, particularly its influence on Th17 cell differentiation and airway inflammation.
- Results showed that transferring dendritic cells infected with Der p2 rBCG led to reduced airway inflammation and mucin production, effectively inhibiting excessive Th17 responses compared to traditional BCG.
- The research highlights the importance of dendritic cells in rBCG's immunoregulatory functions and provides insight into the cellular mechanisms behind its potential as an asthma treatment.
Article Abstract
Background And Objective: Previous studies have demonstrated that our recombinant bacille Calmette-Guerin (rBCG), which expresses Der p2 in house dust mite (Der p2 rBCG) suppresses asthmatic airway inflammation by regulating the phenotype and function of dendritic cells (DC) and reprogramming T helper (Th) 0 cell differentiation into different T cell (Th1/Th2/Treg) subtypes. However, the exact role of Der p2 rBCG in reprogramming Th17 differentiation and the relevant mechanisms are not known. The aim of this study was to examine whether Der p2 rBCG-mediated inhibition of allergic airway inflammation is mediated by regulating Th17 differentiation in a murine asthma model.
Methods: Primary mouse bone marrow-derived dendritic cells (BMDC) were infected with Der p2 rBCG and adoptively transferred to Der p2-intranasally sensitized mice. The role of Der p2 rBCG-BMDC on the regulation of airway inflammation and Th17 cell differentiation was assessed.
Results: Adoptive transfer of Der p2 rBCG-BMDC suppressed airway inflammation and mucin secretion. Der p2 rBCG-BMDC inhibited excessive Th17 immune responses but not BCG-BMDC. Furthermore, Der p2 rBCG decreased jagged-2 and increased delta-like-4 expressions on BMDC to a greater extent than BCG.
Conclusions: These findings suggest that DC plays a key role in Der p2 rBCG-induced immunoregulation. Der p2 rBCG also displayed a potent inhibitory effect on Th17 differentiation, and these findings increase our understanding of the cellular basis of Der p2 BCG-mediated inhibition of asthma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/resp.12198 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decrease of IL-5 Production by Naive T Cells Cocultured with IL-18-Producing BCG-Pulsed Dendritic Cells from Patients Allergic to House Dust Mite.
Vaccines (Basel)
March 2021
Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland.
The only currently available anti-tuberculosis vaccine, Bacillus Calmette-Guérin (BCG), has been reported to also protect against unrelated diseases, including inflammatory diseases such as allergic asthma. Recombinant BCG strains that produce IL-18 have been shown to enhance Th1 responses over non-recombinant BCG and to reduce IL-5 production and bronchoalveolar eosinophilia in mice. However, their ability to decrease the immune polarization of human Th2 cells is not known.
View Article and Find Full Text PDFDer p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma.
Respirology
January 2014
Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Article Synopsis
- The study investigates the effects of recombinant bacille Calmette-Guerin (rBCG) that expresses Der p2 on asthma, particularly its influence on Th17 cell differentiation and airway inflammation.
- Results showed that transferring dendritic cells infected with Der p2 rBCG led to reduced airway inflammation and mucin production, effectively inhibiting excessive Th17 responses compared to traditional BCG.
- The research highlights the importance of dendritic cells in rBCG's immunoregulatory functions and provides insight into the cellular mechanisms behind its potential as an asthma treatment.
Der p 2 recombinant bacille Calmette-Guérin targets dendritic cells to inhibit allergic airway inflammation in a mouse model of asthma.
Respiration
May 2013
Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Article Synopsis
- Researchers studied a recombinant bacille Calmette-Guérin (rBCG) vaccine expressing the Der p 2 allergen, which showed promise in reducing asthma symptoms by manipulating immune responses.
- The study aimed to investigate the role of dendritic cells (DCs) in this process and involved transferring DCs from vaccinated mice to other mice to assess their impact on airway inflammation.
- Results indicated that the transferred DCs significantly reduced asthmatic responses and promoted the development of regulatory T cells, suggesting that DCs are crucial for the rBCG's anti-asthma effects, especially through enhanced recruitment of certain DC subtypes.
Suppression of allergic airway inflammation in a mouse model by Der p2 recombined BCG.
Immunology
September 2009
Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Article Synopsis
- Allergic asthma is driven by T helper (Th)2 cell immune responses, and new immunotherapy strategies aim to shift these responses towards Th1 to alleviate symptoms.
- Previous studies demonstrated that a genetically modified form of bacille Calmette-Guerin (rBCG) could induce such a shift in naive mice, but its effectiveness in reducing allergic airway inflammation needed further investigation.
- The current research showed that rBCG significantly reduced airway inflammation and related symptoms in allergic mice by promoting a Th1 response and enhancing regulatory T cells that suppress Th2 cells, indicating its potential as an effective asthma treatment.
[The effects of rBCG expressing Der p2 in the form of lipoprotein on murine immune response].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2005
Department of Respiratory Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Aim: To investigate the effects of rBCG vaccination containing foreign antigen Der p2 in the form of lipoprotein on murine immune response.
Methods: 6 to 8 weeks old and newborn BALB/c mice were vaccined intraperitoneally with 10(6) CFU rBCG or BCG. At the same time, the control group was injected with saline.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!