AI Article Synopsis
- Neutralizing antibodies are crucial for protection against mumps virus, but measuring them is complicated and time-consuming, leading to reliance on ELISAs to check for mumps-specific IgG levels.
- There’s a poor correlation between these IgG measurements and actual mumps neutralization, which indicates the need for better ELISA methods that match neutralization outcomes.
- The study showed that responses to different mumps proteins might explain the inconsistencies in serologic tests, and that some people who test positive might still have low levels of neutralizing antibodies, potentially overstating their immunity.
Article Abstract
Neutralizing antibodies are assumed to be essential for protection against mumps virus infection, but their measurement is labor- and time-intensive. For this reason, enzyme-linked immunosorbent assays (ELISAs) are typically used to measure mumps-specific IgG levels. However, since there is poor correlation between mumps neutralization titers and ELISAs that measure the presence of mumps-specific IgG levels, ELISAs that better correlate with neutralization are needed. To address this issue, we measured mumps antibody levels by plaque reduction neutralization, by a commercial ELISA (whole-virus antigen), and by ELISAs specific for the mumps nucleoprotein and hemagglutinin. The results indicate that differences in the antibody response to the individual mumps proteins could partially explain the lack of correlation among various serologic tests. Furthermore, the data indicate that some seropositive individuals have low levels of neutralizing antibody. If neutralizing antibody is important for protection, this suggests that previous estimates of immunity based on whole-virus ELISAs may be overstated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957677 | PMC |
| http://dx.doi.org/10.1128/CVI.00621-13 | DOI Listing |
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