Monodisperse Fe3O4 magnetic nanoparticles (NPs) were prepared under facile solvothermal conditions and successively functionalized with silica and Au to form core/shell Fe3O4@silica@Au NPs. Furthermore, the samples were used as matrix to construct a glucose sensor based on glucose oxidase (GOD). The immobilized GOD retained its bioactivity with high protein load of 3.92×10(-9) mol·cm(-2), and exhibited a surface-controlled quasi-reversible redox reaction, with a fast heterogeneous electron transfer rate of 7.98±0.6 s(-1). The glucose biosensor showed a broad linear range up to 3.97 mM with high sensitivity of 62.45 μA·mM(-1) cm(-2) and fast response (less than 5s).
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