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Dissecting Cytophagalysin: Structural and Biochemical Studies of a Bacterial Pappalysin-Family Metallopeptidase.
Biomolecules
December 2024
Synthetic Structural Biology Group, Molecular Biology Institute of Barcelona (IBMB), Spanish National Research Council (CSIC), 08028 Barcelona, Spain.
Cytophaga is a genus of Gram-negative bacteria occurring in soil and the gut microbiome. It is closely related to pathogenic spp. that cause severe diseases in fish.
View Article and Find Full Text PDFModeling, synthesis and in vitro testing of peptides based on unusual amino acids as potential antibacterial agents.
Biomed Khim
December 2024
Scientific and Production Center "Armbiotechnology" NAS RA, Yerevan, Armenia; Yerevan State University, Yerevan, Armenia.
Currently non-protein amino acids and synthetic peptides are widely used as blocks in drug design. Many proteases are of great interest for pharmacology due to their key role in various pathologies. Bacterial collagenase (EC 3.
View Article and Find Full Text PDFRole of Metalloproteinases in Adhesion to Radicular Dentin: A Literature Review.
Materials (Basel)
November 2024
Maestría en Rehabilitación Oral Avanzada, Facultad de Odontología, Universidad Autónoma de Sinaloa, Sinaloa 80040, Mexico.
Introduction: Root dentin is a porous and complex dental surface that may have irregularities and deposits of organic material. To achieve an effective bond between restorative materials and root dentin, it is necessary that the restorative materials adhere intimately to the dentin surface. Metalloproteinases (MMPs) are a group of proteolytic enzymes that perform an important role in degrading the extracellular matrix and remodeling connective tissue.
View Article and Find Full Text PDFPurification and characterization of a protease produced by submerged fermentation: Ultrasound-enhanced collagenolytic protease from Streptomyces parvulus.
Int J Biol Macromol
December 2024
Laboratory of Advances in Protein Biotechnology (LABIOPROT), Institute of Biological Sciences, University of Pernambuco - UPE, Brazil. Electronic address:
Proteases are a large group of enzymes in high demand due to their wide and different biotechnological applications mainly in the biomedical field. Ultrasound (US) has been used successfully in several Bioprocesses in biotechnology, such as in the upregulation of enzymatic hydrolysis (biocatalysis). The objective of this work was to purify an enzyme from Streptomyces parvulus and to characterize it through physic-chemical applications including ultrasound effect.
View Article and Find Full Text PDFModeling collagen fibril degradation as a function of matrix microarchitecture.
Soft Matter
November 2024
Department of Mechanical and Aerospace Engineering, University of California San Diego, CA 92093, USA.
Collagenolytic degradation is a process fundamental to tissue remodeling. The microarchitecture of collagen fibril networks changes during development, aging, and disease. Such changes to microarchitecture are often accompanied by changes in matrix degradability.
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