Two-photon fluorescence microscopy (2PM) enables scientists in various fields including neuroscience, embryology, and oncology to visualize and tissue morphology and physiology at a cellular level deep within scattering tissue. However, tissue scattering limits the maximum imaging depth of 2PM within the mouse brain to the cortical layer, and imaging subcortical structures currently requires the removal of overlying brain tissue or the insertion of optical probes. Here we demonstrate non-invasive, high resolution, imaging of subcortical structures within an intact mouse brain using three-photon fluorescence microscopy (3PM) at a spectral excitation window of 1,700 nm. Vascular structures as well as red fluorescent protein (RFP)-labeled neurons within the mouse hippocampus are imaged. The combination of the long excitation wavelength and the higher order nonlinear excitation overcomes the limitations of 2PM, enabling biological investigations to take place at greater depth within tissue.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864872 | PMC |
http://dx.doi.org/10.1038/nphoton.2012.336 | DOI Listing |
J Child Psychol Psychiatry
December 2024
Department of Radiology and Huaxi MR Research Center (HMRRC), Functional and Molecular Imaging Key Laboratory of Sichuan Province, West China Hospital, Sichuan University, Chengdu, China.
Background: Neuroimaging studies have identified brain structural and functional alterations in adolescents with major depressive disorder (MDD); however, the results are inconsistent, and whether patients exhibit spatially convergent structural and functional brain abnormalities remains unclear.
Methods: We conducted voxel-wise meta-analysis of voxel-based morphometry (VBM) and resting-state functional studies, respectively, to identify regional gray matter volume (GMV) and brain activity alterations in adolescent MDD patients. Multimodal analysis was performed to examine the overlap of regional GMV and brain activity alterations.
BMC Neurosci
December 2024
The Clinical Hospital of Chengdu Brain Science Institute, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, P.R. China.
Background: Parkinson's disease (PD) is a progressive neurodegenerative disease associated with functional and structural alterations beyond the nigrostriatal dopamine projection. However, the structural-functional (SC-FC) coupling changes in combination with subcortical regions at the network level are rarely investigated in PD.
Methods: SC-FC coupling networks were systematically constructed using the structural connectivity obtained by diffusion tensor imaging and the functional connectivity obtained by resting-state functional magnetic resonance imaging in 53 PD and 72 age- and sex-matched healthy controls (HCs).
Schizophrenia (Heidelb)
December 2024
Skolkovo Institute of Science and Technology, Moscow, Russia.
Numerous brain imaging studies have reported white matter alterations in schizophrenia, but the lipidome analysis of the corresponding tissue remains incomplete. In this study, we investigated the lipidome composition of six subcortical white matter regions corresponding to major axonal tracks in both control subjects and schizophrenia patients. All six regions exhibited a consistent pattern of quantitative lipidome alterations in schizophrenia, involving myelin-forming and mitochondria associated lipid classes.
View Article and Find Full Text PDFBrain Res
December 2024
Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:
Introduction: Prior researches have reported abnormal changes of thalamus in patients with subcortical ischemic vascular disease (SIVD), which was usually analyzed as a whole. However, it was currently unclear whether the structure, function and connectivity of thalamic subregions were differentially affected by this disease and affected different cognitive functions.
Methods: This study recruited 30 SIVD patients with cognitive impairment (SIVD-CI), 30 SIVD patients with cognitive unimpaired (SIVD-CU) and 32 normal controls.
Neuroimage Clin
December 2024
Department of Radiology, Mayo Clinic, Rochester, MN, USA.
Progressive supranuclear palsy (PSP) can present with different clinical variants which show distinct, but partially overlapping, patterns of neurodegeneration and tau deposition in a network of regions including cerebellar dentate, superior cerebellar peduncle, midbrain, thalamus, basal ganglia, and frontal lobe. We sought to determine whether disruptions in functional connectivity within this PSP network measured using resting-state functional MRI (rs-fMRI) differed between PSP-Richardson's syndrome (PSP-RS) and the cortical and subcortical clinical variants of PSP. Structural MRI and rs-fMRI scans were collected for 36 PSP-RS, 25 PSP-cortical and 34 PSP-subcortical participants who met the Movement Disorder Society PSP clinical criteria.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!