The presence of Cryptococcus spp. has been reported in Mexico's capital city; however, to our knowledge there are no reports of its presence in the state of Nuevo León located in northeast Mexico. This is presumed to be because the hot and dry climate in this region does not favor cryptococcal proliferation. This study confirmed the presence of C. neoformans and C. albidus in 20% (10/50) of randomly selected fecal samples of pigeons (Columba livia) in the Monterrey metropolitan area. The presence of this yeast in the state of Nuevo León is proof of its adaptation to the typically hot climate of the area and is consistent with recent reviews of cryptococcosis cases in several local hospitals. The two species were identified and characterized through microbiological tests and molecular identification by DNA extraction and PCR amplification of highly conserved 18S ribosomal DNA using ITS1 and ITS2 as target regions. The PCR products were sequenced and compared with those reported in GenBank.
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http://dx.doi.org/10.1186/2193-1801-2-632 | DOI Listing |
Arch Peru Cardiol Cir Cardiovasc
December 2024
Departamento de Cardiología Clínica. Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, Ciudad De México, Mexico. Departamento de Cardiología Clínica Instituto Nacional de Cardiología Ignacio Chávez Ciudad De México Mexico.
Infective endocarditis is a disease that affects mainly the endocardial surface of the heart and cardiac valves (native or prosthetic). The main risk factors for developing infective endocarditis are male sex, older age, intracardiac shunts, prosthetic valves, rheumatic, and congenital heart disease, intracardiac devices, intravenous drugs use, immunosuppression, and hemodialysis. Streptococci and Staphylococci spp.
View Article and Find Full Text PDFMycopathologia
December 2024
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), University Miguel Hernández, Campus of San Juan de Alicante, 03550, Alicante, Spain.
Pulmonary surfactant, the primary substance lining the epithelium of the human Lower Respiratory Tract (LRT), is rich in lipids, with dipalmitoyl-phosphatidylcholine (DPPC) being the most abundant. Although surfactants are known to have antifungal activity against some yeast species, the significant presence of species like Malassezia restricta in the lung mycobiome suggests that these yeasts may exhibit some level of lipo-tolerance or even lipo-affinity for pulmonary lipids. This study explored the affinity and tolerance of yeasts, identified as significant members of the lung microbiome, to pulmonary lipids through culture-based methods.
View Article and Find Full Text PDFSci Rep
December 2024
Clinical Microbiology Laboratory, National Reference Center for Mycosis, Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, Liege, Belgium.
We evaluated the prevalence of serum and meningeal cryptococcosis in asymptomatic outpatients with advanced HIV disease (CD4 < 200 cells/mm3) in a cross-sectional screening context in Kinshasa clinics (DRC). Lumbar puncture (LP) was performed in patients with positive serum cryptococcal antigen (CrAg) test, and Cryptococcus spp. isolated from cerebrospinal fluid (CSF) were identified by MALDI-TOF-MS, and characterized using serotyping-PCR, ITS-sequencing and multilocus sequence typing (MLST).
View Article and Find Full Text PDFCan J Microbiol
January 2025
Postgraduation Program in Cellular and Molecular Biology, Federal University of Rio Grande do Sul - Porto Alegre, RS 91501-970, Brazil.
Future Microbiol
January 2025
Laboratory of Mycology & Center of Proteomics, Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, 14800-903, Brazil.
This work aimed to test peptides against the planktonic and biofilm form of spp. and toxicity using three-dimensional (3D) cells characterized and evaluate toxicity in . Susceptibility tests were conducted on the planktonic form and biofilm formation.
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