AI Article Synopsis
- * Researchers cultured and analyzed BMSCs to assess their gene expression and functionality, focusing on their ability to migrate and adhere, as well as their structural integrity.
- * Findings indicate that BMSCs can express specific markers, migrate effectively, and contribute to vascular repair by differentiating into ECs, suggesting they could have therapeutic benefits for treating vascular diseases.
Article Abstract
Objectives: Recent findings suggest that in response to repair-to-injury bone marrow mesenchymal stem cells (BMSCs) participate in the process of angiogenesis. It is unclear what role BMSCs play in the structure of the vessel wall. In present study, we aimed to determine whether BMSCs had the capacity of endothelial cells (ECs).
Methods: BMSCs were separated and cultured. FACS and RT-PCR analysis confirmed the gene expression phenotype. The capacity of migration and adhesion and the ultrastructure of BMSCs were examined. The effect of BMSCs transplantation on the vascular repair was investigated in a murine carotid artery-injured model.
Results: BMSCs could express some markers and form the tube-like structure. The migration and adhesion capacity of BMSCs increased significantly after stimulated. In addition, BMSCs had the intact cell junction. In vivo the local transfer of BMSCs differentiated into neo-endothelial cells in the injury model for carotid artery and contributed to the vascular remodeling.
Conclusion: These results showed that BMSCs could contribute to neointimal formation for vascular lesion and might be associated with the differentiation into ECs, which indicated the important therapeutic implications for vascular diseases.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857273 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082743 | PLOS |
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