Energy homeostasis is regulated by endocrine factors. The concentration of relaxin-3 in serum is related to body mass index. However, relaxin-3 is found only in the brain and testis. In this study, we examined the expression of relaxin- 3 in adipose tissue and its effects on adipogenesis. The expression of relaxin-3 was determined using RT-PCR, a relaxin- 3 C-peptide-specific radioimmunoassay, specifically in the stromal-vascular fraction (SVF) cells rather than adipocytes. The release of C-peptide was regulated by glucose concentration in the SVF cells. However, the differentiated adipocytes did not express relaxin-3. In glucose perfusion experiments, C-peptide was released in response to high glucose concentrations in the mesenteric perfusate, opposite to insulin release. Additionally, GPCR135 mRNA was expressed in adipocytes. Relaxin-3 increased triglycerides in adipocytes and decreased lipase activity. The present study showed that relaxin-3 is secreted from SVF cells and that it regulates lipid accumulation in adipocytes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/0929866520666131217101424 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tissue Engineering 3D-Printed Scaffold Using Allograft/Alginate/Gelatin Hydrogels Coated With Platelet-Rich Fibrin or Adipose Stromal Vascular Fraction Induces Osteogenesis In Vitro.
J Cell Physiol
December 2024
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Incorporating autologous patient-derived products has become imperative to enhance the continually improving outcomes in bone tissue engineering. With this objective in mind, this study aimed to evaluate the osteogenic potential of 3D-printed allograft-alginate-gelatin scaffolds coated with stromal vascular fraction (SVF) and platelet-rich fibrin (PRF). The primary goal was to develop a tissue-engineered construct capable of facilitating efficient bone regeneration through the utilization of biomaterials with advantageous properties and patient-derived products.
View Article and Find Full Text PDFTherapeutic Potential of Stromal Vascular Fraction in Enhancing Wound Healing: A Preclinical Study.
Aesthetic Plast Surg
December 2024
Department of Medical Biochemistry, Faculty of Medicine, Istinye University, Istanbul, Turkey.
Background: Adipose tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration. It can also give rise to many multipotent adipose-derived stromal cells. SVF is the population of cells obtained from mechanical or enzymatic digestion of lipoaspirate with no necessity for cell culture or expansion.
View Article and Find Full Text PDFSafety of adipose-derived stromal vascular fraction cells to treat Parkinson's disease.
Parkinsonism Relat Disord
November 2024
Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA. Electronic address:
Neuroinflammation is a significant correlate of Parkinson's Disease (PD), with positron emission tomography showing microglial activation early in the PD process and post-mortem tissue containing reactive microglia. Adipose-derived (AD) stromal vascular fraction (SVF) cells have been shown to respond to pro-inflammatory conditions with secretion of anti-inflammatory paracrine factors. This pilot clinical trial was to examine the safety and clinical response using autologous ADSVF to treat PD.
View Article and Find Full Text PDFSafety and efficacy of autologous adipose-derived stem cells for knee osteoarthritis in the elderly population: A systematic review.
J Clin Orthop Trauma
December 2024
Department of Orthopaedic and Trauma Surgery, Università Campus Bio-Medico di Roma, Via Alvaro del Portillo, 21, 00128, Roma, Italy.
Introduction: Osteoarthritis (OA) is a progressive joint disease, and over 240 million people suffer from symptomatic OA, primarily in the knee, and mainly affects the elderly population over 65. A combination of different risk factors leads to biological changes in the microenvironments of the joints, causing cartilage overload and chondrocyte aging. Adipose-derived MSCs (ADSCs) are demonstrated to improve joint environments with an effective therapy for Knee OA.
View Article and Find Full Text PDFSingle-cell view and a novel protective macrophage subset in perivascular adipose tissue in T2DM.
Cell Mol Biol Lett
December 2024
Department of Cardiology, State Key Laboratory for Innovation and Transformation of Luobing Theory, Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, Qilu Hospital of Shandong University, Jinan, 250012, China.
Background: Vasculopathy underlies diabetic complications, with perivascular adipose tissue (PVAT) playing crucial roles in its development. However, the changes in the cellular composition and function of PVAT, including the specific cell subsets and mechanisms implicated in type 2 diabetes mellitus (T2DM) vasculopathy, remain unclear.
Methods: To address the above issues, we performed single-cell RNA sequencing on the stromal vascular fraction (SVF) of PVAT from normal and T2DM rats.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!