AI Article Synopsis
- A study was conducted to investigate how HIF-1α affects the growth of retinoblastoma cells through siRNA knockdown followed by proliferation assays.
- Results showed that knocking down HIF-1α significantly reduced the proliferation of retinoblastoma cells, indicating its role in promoting cell survival and growth.
- Although attempts to stabilize HIF-1α using CoCl2 in retinoblastoma cells were made, these efforts were not successful.
Article Abstract
In order to better understand the role of HIF-1α in the proliferation of the retinoblastoma cells, a siRNA knockdown of HIF-1α followed by a proliferation assay was performed. Further sequencing was then carried out in order to assess knockdown efficiency and expression of HIF-1α. Upregulation of HIF-1α gene expression in CoCl2-treated retinoblastoma cells was demonstrated via melting curve analysis from PCR tests and was further analyzed using western blot and densitometry analysis. Reduction of HIF-1α expression in retinoblastoma, post HIF-1α knockdown, was observed after siRNA transfection into Y-79 cells. Knockdown of HIF-1α resulted in a significant decrease in proliferation thereby demonstrating that HIF-1α is involved in promoting survival and proliferation in retinoblastoma cells. Stabilization of HIF-1α in retinoblastoma cells using CoCl2 was unsuccessful.
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| http://dx.doi.org/10.1007/s12253-013-9728-8 | DOI Listing |
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