Hypoxia-inducible factor 1α (HIF1α) is a transcription factor involved in the growth, invasion and metastasis of malignant tumors. Glycogen synthase kinase 3 beta (GSK3β) is a protein kinase involved in a variety of signaling pathways, such as the Wnt and NF-κB pathways; this kinase can affect tumor progress through the regulation of transcription factor expression and apoptosis. Recent studies showed that GSK3β was involved in the expression of HIF1α. However, the effect of GSK3β on HIF1α expression in osteosarcoma cells remains unknown. To understand the relationship between GSK3β and HIF1α comprehensively, small RNA interference techniques, Western blot analyses, quantitative real-time PCR analyses and luciferase assays were used in our study. Experimental data revealed that inhibition of GSK3β could increase HIF1α protein levels and expression of its target genes by increasing the stability of the HIF1α mRNA, not by affecting the HIF1α protein stability, and that this process could be mediated by nucleolin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2013.12.020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Down to size: Exploring the influence of plastic particle Dimensions on physiological and nervous responses in early-stage zebrafish.
Environ Pollut
June 2024
Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, University of Namur, Rue de Bruxelles, 61-B-5000, Namur, Belgium.
The chorion is the first protective barrier set to prevent numerous pollutants from damaging the developing embryo. However, depending on their size, some nanoplastics (NPs) can pass through this barrier and reach the embryo, while all microplastics (MPs) remain on the outside. This study brings a straight approach to compare MPs and NPs, and assess their direct and indirect effects on zebrafish embryos and larvae.
View Article and Find Full Text PDFHypoxia inducible factor 1-α is minimally involved in determining the time domains of the hypoxic ventilatory response in adult zebrafish (Danio rerio).
Respir Physiol Neurobiol
December 2021
Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, K1N6N5 Canada.
In the current study, adult zebrafish (Danio rerio) were exposed to 72 h hypoxia (90 mmHg) to assess the time domains of the hypoxia ventilatory response (HVR) and the consequence on a subsequent more severe (40 mmHg) bout of acute hypoxia. Experiments were performed on wild-type fish and mutants in which one or both paralogs of hypoxia inducible factor-1α (hif-1α) were knocked out. Although there were subtle differences among the wild-type and knockout fish, resting f was reestablished after 2-8 h of continuous hypoxia in both groups, a striking example of hypoxic ventilatory decline (HVD).
View Article and Find Full Text PDFDoes hypoxia-inducible factor 1α play a role in regulating cutaneous oxygen flux in larval zebrafish (Danio rerio)?
J Comp Physiol B
July 2021
Department of Biology, University of Ottawa, Gendron Hall, 30 Marie Curie Private, Ottawa, ON, K1N 6N5, Canada.
Previous studies have demonstrated that hypoxia tolerance is improved in zebrafish (Danio rerio) larvae after prior exposure to lowered ambient O levels. Such improved hypoxia performance was attributed in part, to increased levels of hypoxia-inducible factor 1α (Hif-1α) exerting downstream effects on various physiological processes including promotion of trunk skin angiogenesis. Since O uptake ([Formula: see text]) in larvae is facilitated largely by O diffusion across the skin, enhanced cutaneous vascularization is expected to enhance [Formula: see text] during hypoxia and thus contribute to improved hypoxia tolerance.
View Article and Find Full Text PDFSpecific cyprinid HIF isoforms contribute to cellular mitochondrial regulation.
Sci Rep
October 2020
College of Fisheries, National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan, 430070, China.
Hypoxia-inducible factor 1 (HIF-1) functions as a master regulator of the cellular response to hypoxic stress. Two HIF-1α paralogs, HIF-1αA and HIF-1αB, were generated in euteleosts by the specific, third round of genome duplication, but one paralog was later lost in most families with the exception of cyprinid fish. How these duplicates function in mitochondrial regulation and whether their preservation contributes to the hypoxia tolerance demonstrated by cyprinid fish in freshwater environments is not clear.
View Article and Find Full Text PDFHypoxia inducible factor-1 knockout does not impair acute thermal tolerance or heat hardening in zebrafish.
Biol Lett
July 2020
Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, ON Canada, K1N 6N5.
Article Synopsis
- Heat hardening in fish refers to the ability to increase thermal tolerance after exposure to high temperatures, helping them adapt to extreme environments.
- The study focused on zebrafish lacking specific hypoxia-inducible factors (Hif-1), hypothesizing that these fish would show reduced thermal tolerance and diminished heat hardening.
- Contrary to expectations, both Hif-1 knockout and wild-type zebrafish showed similar thermal tolerance and heat hardening abilities, indicating that hypoxia tolerance and thermal tolerance are not functionally linked.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!