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Preparation and Characterization of C-Reactive Protein Dual-Particle Latex-Enhanced Immunoturbidimetric Reagents.
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Department of Laboratory Medicine and Center of Infectious Diseases and Pathogen Biology, The First Hospital of Jilin University, Changchun 130021, China.
This study aims to couple C-reactive protein (CRP) antibodies onto latex spheres of 2 different sizes to enhance the accuracy and sensitivity of CRP detection. Furthermore, it seeks to establish a robust methodological framework crucial for advancing the development of latex-enhanced immunoturbidimetric detection reagents. CRP, an acute-phase protein, rapidly elevates in response to infections or tissue damage.
View Article and Find Full Text PDFHeat Treatment of Hazelnut Allergens Monitored by Polyclonal Sera and Epitope Fingerprinting.
Foods
December 2024
Department Diagnostics, Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstr. 1, 04103 Leipzig, Germany.
Hazelnuts are frequently involved in IgE-mediated reactions and are the main cause of nut allergies in Europe. Most food products are processed before human consumption. Food processing can modify the structure, properties, and function of proteins, and as a result, the IgE-binding capacity of allergens can be affected.
View Article and Find Full Text PDFPreparation of polyclonal antibody to CHOP protein and its application in heat stress of chickens.
Int J Biol Macromol
December 2024
Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address:
Heat stress (HS) is a stress response of organisms to temperature changes, which can result in organ damage and increased chicken mortality in high-temperature environments. The CHOP protein, also known as GADD 135, plays a crucial role in endoplasmic reticulum stress. However, there are fewer studies related to whether CHOP proteins are involved in heat stress-induced organ damage.
View Article and Find Full Text PDFEstablishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen.
Acta Trop
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Department of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, PR China; inxiang Key Laboratory of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. Electronic address:
Background: Trichomonas vaginalis (T. vaginalis) is a common sexually transmitted parasite that colonizes the human urogenital tract. Programmed and precise detection of T.
View Article and Find Full Text PDFIn vivo study of nano chitosan beads-based ELISA versus traditional sandwich ELISA for the early diagnosis of trichinosis.
Exp Parasitol
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Medical Parasitology, Faculty of Medicine, Beni-Suef University, Egypt. Electronic address:
Human trichinosis is a serious foodborne parasitic zoonosis. Diagnosing human trichinosis is usually difficult due to the nonspecific clinical picture and the limited effectiveness of serological tests in acute infections. While ELISA can detect circulating Trichinella antigens, aiding in early diagnosis, its sensitivity may be low.
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