We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10517-013-2288-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Osteoarthritis, a major global cause of pain and disability, is driven by the irreversible degradation of hyaline cartilage in joints. Cartilage tissue engineering presents a promising therapeutic avenue, but success hinges on replicating the native physiological environment to guide cellular behavior and generate tissue constructs that mimic natural cartilage. Although electrical stimulation has been shown to enhance chondrogenesis and extracellular matrix production in 2D cultures, the mechanisms underlying these effects remain poorly understood, particularly in 3D models.
View Article and Find Full Text PDFEffect of nanogold incorporation into polymethyl methacrylate denture bases on microbial activity in implant-retained mandibular overdentures.
Int J Implant Dent
January 2025
Lecturer at removable prosthodontic department, Faculty of dental medicine for Girls, Al-Azhar University, Cairo, Egypt.
Purpose: In this randomized clinical trial, we examined the incorporation of nanogold particles into polymethyl methacrylate denture bases and compared these modified bases with conventional ones in mandibular implant-retained overdentures, focusing on microbiological growth and adhesion characteristics.
Methods: In this study, twenty-two male patients who were completely edentulous participated in a rehabilitation program involving mandibular overdentures retained by two dental implants placed in the canine area. The subjects were categorized into two equal groups, each comprising eleven patients.
Cyclic Voltarefractometry of Single TiO Nanoparticles in Large Ensembles in Nonaqueous Electrolyte.
Anal Chem
January 2025
Nanobiotechnology Department of the Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitaetsplatz 1, Senftenberg 01968, Brandenburg, Germany.
Single nanoparticle (NP) cyclic voltarefractometry (CVR), realized as wide-field surface plasmon resonance microscopy (SPRM) in combination with potential cycling, has been proposed and applied to the in situ study of TiO NPs. Electrochemical activity of TiO is mainly observed outside the electrochemical stability window of water. Therefore, the response of individual anatase (a-TiO) and rutile (r-TiO) NPs adsorbed on a gold layer was studied in 0.
View Article and Find Full Text PDFRecent trends and impact of localized surface plasmon resonance (LSPR) and surface-enhanced Raman spectroscopy (SERS) in modern analysis.
J Pharm Anal
November 2024
Department of Pharmaceutical Analysis, ISF College of Pharmacy Moga, 142001, Punjab, India.
An optical biosensor is a specialized analytical device that utilizes the principles of optics and light in bimolecular processes. Localized surface plasmon resonance (LSPR) is a phenomenon in the realm of nanophotonics that occurs when metallic nanoparticles (NPs) or nanostructures interact with incident light. Conversely, surface-enhanced Raman spectroscopy (SERS) is an influential analytical technique based on Raman scattering, wherein it amplifies the Raman signals of molecules when they are situated near specific and specially designed nanostructures.
View Article and Find Full Text PDFNanoparticle trends and hotspots in lung cancer diagnosis from 2006-2023: a bibliometric analysis.
Front Oncol
December 2024
Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China.
Background: Lung cancer possesses the highest incidence and mortality rates among malignancies globally. Despite substantial advancements in oncology, it is frequently diagnosed at an advanced stage, resulting in a poor prognosis. Over recent decades, the swift progress of nanotechnology has precipitated the extensive utilization of nanomaterials as carriers in cancer diagnosis and therapy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!