AI Article Synopsis
- This study examined whether nucleotide supplementation could reduce ethanol-related developmental damage in mouse embryos using whole embryo culture and midbrain cell micromass culture models.
- Ethanol exposure led to various malformations in embryos, but nucleotide supplementation improved growth metrics, with the best results seen at a dosage of 4.00 mg/L.
- The research suggests that nucleotides could help prevent ethanol-induced birth defects, but highlights the need for careful dosage considerations.
Article Abstract
The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844198 | PMC |
| http://dx.doi.org/10.1155/2013/204187 | DOI Listing |
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