Small molecule fluorescent probes offer significant advantages over conventional antibody and fluorescent protein labeling techniques. Here we present and , dyes that label live microglia specifically. They may be applied to the isolation and imaging of live microglia when investigating their role in neuroinflammatory diseases.
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http://dx.doi.org/10.1039/c3cc45715j | DOI Listing |
J Ethnopharmacol
December 2024
Yunnan Characteristic Plant Extraction Laboratory, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, P. R. China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, 650201, P.R. China. Electronic address:
Ethnopharmacological Relevance: Pachysandra axillaris Franch., a traditional herbal medicine in Yunnan, has been used to treat traumatic injuries and stomach ailments, some of which were related to microbial infections in conventional applications, but, to the best of our knowledge, the antifungal bioactivity of this plant and its main antifungal components have not been previously reported.
Aim Of The Study: To identify the antifungal compounds of P.
Methods Mol Biol
December 2024
Department of Biochemistry and Molecular Biology & The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
We present protocols for using an optogenetic tool called LILAC for actin imaging. LILAC is a light-controlled version of Lifeact that uses the Avena sativa LOV2 (AsLOV2) domain. By significantly reducing Lifeact's affinity for the cytoskeleton in the dark, LILAC reduces concentration-dependent negative side effects while enabling new image processing methods.
View Article and Find Full Text PDFAnal Sci
December 2024
NHC Key Laboratory for Engineering Control of Dust Hazard, National Center for Occupational Safety and Healthy, NHC, Beijing, 102308, China.
We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP.
View Article and Find Full Text PDFAnal Chem
December 2024
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
To answer the call for effective and timely intervention in cardiovascular diseases (CVDs), the development of fluorescent probes that can precisely identify atherosclerotic plaques, the root cause of various fatal CVDs, is highly desirable but remains a great challenge. Herein, by integrating bis(trifluoromethyl)benzyl and phenothiazine into the coumarin matrix, a robust fluorescent probe, NOR1, has been developed. NOR1 responds sequentially to lipid droplets (LDs) and HClO via fluorescence turn-on and ratiometric readouts, respectively, with a fast response rate (within 70 s for LDs and 80 s for HClO), excellent sensitivity (detection limit: 0.
View Article and Find Full Text PDFLuminescence
December 2024
Department of Chemistry and Chemical Engineering, College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, China.
Herein, a kind of N-doped fluorescent carbon dots (N-CDs) were prepared by using melamine and carboxymethyl cellulose (CMC) as precursors through a straightforward hydrothermal method. The designed sensor displayed a uniform nanoscale distribution, excellent hydrophilicity, and strong fluorescence emission with a fluorescence quantum yield of 37.98%.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!