AI Article Synopsis
- Prebiotics are substances that boost the growth of good bacteria in the gut, with galactooligosaccharides (GOS) being a key example that encourages bifidobacteria growth.
- Current understanding of how bifidobacteria consume GOS is limited, prompting research to identify which specific GOS structures are most effective.
- The study developed a method combining various scientific techniques to analyze GOS isomers, revealing that different bifidobacteria species prefer specific GOS, which could enhance their targeted use in infant formula.
Article Abstract
Prebiotics are nondigestible substrates that stimulate the growth of beneficial microbes in the human intestine. Galactooligosaccharides (GOS) are food ingredients that possess prebiotic properties, in particular, promoting the growth of bifidobacteria in situ. However, precise mechanistic details of GOS consumption by bifidobacteria remain poorly understood. Because GOS are mixtures of polymers of different lengths and linkages, there is interest in determining which specific structures provide prebiotic effects to potentially create better supplements. This paper presents a method comprising porous graphitic carbon separation, isotopic labeling, and mass spectrometry analysis for the structure-specific analysis of GOS isomers and their bacterial consumption rate. Using this strategy, the differential bacterial consumption of GOS by the bifidobacteria species Bifidobacterium longum subsp. infantis, Bifidobacterium animalis subsp. lactis, and Bifidobacterium adolescentis was determined, indicating that the use of specific GOS isomers in infant formula may provide enrichment of distinct species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912189 | PMC |
| http://dx.doi.org/10.1021/jf403789r | DOI Listing |
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