AI Article Synopsis
- Several initiatives are focused on creating binding reagents for analyzing a wide range of proteins in the proteome.
- Current labeling methods require large amounts of antibodies, need them to be pure, and aren't easily automated, posing limitations for efficient analysis.
- A new protocol has been developed that allows for the purification and modification of antibodies at very low amounts, utilizing magnetic microspheres for automated parallel labeling with biotin or fluorescent dyes.
Article Abstract
There are currently several initiatives that aim to produce binding reagents for proteome-wide analysis. To enable protein detection, visualization, and target quantification, covalent coupling of reporter molecules to antibodies is essential. However, current labeling protocols recommend considerable amount of antibodies, require antibody purity and are not designed for automation. Given that small amounts of antibodies are often sufficient for downstream analysis, we developed a labeling protocol that combines purification and modification of antibodies at submicrogram quantities. With the support of magnetic microspheres, automated labeling of antibodies in parallel using biotin or fluorescent dyes was achieved.
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| http://dx.doi.org/10.1002/pmic.201300283 | DOI Listing |
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