AI Article Synopsis
- * To confirm differentiation, we used dithizone staining and identified the expression of crucial proteins like Pdx1 and insulin through immunofluorescence.
- * The differentiated cells not only produced insulin and C-peptide, but they also demonstrated a response to glucose, indicating they could function like natural pancreatic beta cells.
Article Abstract
In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.
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| http://dx.doi.org/10.3109/21691401.2013.864662 | DOI Listing |
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