AI Article Synopsis
- The article introduces a new, highly sensitive ELISA for monitoring lenalidomide (LND), a drug used to treat multiple myeloma.
- The assay uses a specific polyclonal antibody and LND-BSA conjugate to measure LND levels in plasma, with a detection limit of 0.05 ng/mL and an effective range of 0.1-20 ng/mL.
- With high precision and the ability to analyze about 200 samples per day, the ELISA is valuable for clinical labs in routine therapeutic monitoring and pharmacokinetic studies of LND.
Article Abstract
This article describes, for the first time, a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for therapeutic monitoring and pharmacokinetic studies of lenalidomide (LND), the potent drug for treatment of multiple myeloma (MM). The assay employed a polyclonal antibody that specifically recognizes LND with high affinity, and LND conjugate of bovine serum albumin (LND-BSA) immobilized onto microplate wells as a solid phase. The assay involved a competitive binding reaction between LND, in plasma sample, and the immobilized LND-BSA for the binding sites on a limited amount of the anti-LND antibody. The assay limit of detection was 0.05 ng/mL and the effective working range at relative standard deviations (RSD) of ≤5% was 0.1-20 ng/mL. Analytical recovery of LND from spiked plasma was 100.98 ± 3.05. The precisions of the assay were satisfactory; RSD was 2.96-6.67% and 4.46-7.14%, for the intra- and inter-assay precision, respectively. The procedure is convenient, and one can analyze ∼200 samples per working day, facilitating the processing of large-number batch of samples in clinical laboratories. The proposed ELISA has a great value in routine analysis of LND for its therapeutic monitoring and pharmacokinetic studies.
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| http://dx.doi.org/10.1080/15321819.2013.824898 | DOI Listing |
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