An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30®. Thiolated aptamer specific to OTA was covalently attached to Au nanoparticles via Au-S bonding. The interaction of the aptamer with OTA induced the conformational switch of the aptamer from linear to guanine quadruplex form followed by consolidation of the surface layer and an increase of the charge transfer resistance. The aptasensor makes it possible to detect from 0.1 to 100 nM of OTA (limit of detection: 0.02 nM) in the presence of at least 50 fold excess of ochratoxin B. The applicability of the aptasensor for real sample assay was confirmed by testing spiked beer samples. The recovery of 2 nM OTA was found to be 70% for light beer and 78% for dark beer.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892811 | PMC |
| http://dx.doi.org/10.3390/s131216129 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-invasive ultra-sensitive detection of breast cancer biomarker using cerium nanoparticle functionalized graphene oxide enabled impedimetric aptasensor.
Biosens Bioelectron
January 2025
Industrial Waste Utilization, Nano and Biomaterials, CSIR-Advanced Materials and Processes Research Institute (AMPRI), Hoshangabad Road, Bhopal, 462026, MP, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address:
Epidermal growth factor receptor (EGFR) is a transmembrane protein and a key biomarker implicated in the pathogenesis of breast cancer. Early and precise detection of EGFR is crucial for effective diagnosis, prognosis, and therapeutic intervention. However, conventional EGFR detection techniques, such as biopsy and immunohistochemistry, are often invasive, time-consuming, and limited in sensitivity, highlighting the demand for non-invasive, highly sensitive detection methods.
View Article and Find Full Text PDFOptimized aptamer-based next generation biosensor for the ultra-sensitive determination of SARS-CoV-2 S1 protein in saliva samples.
Int J Biol Macromol
November 2024
Analytical Chemistry Department, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Türkiye.
COVID-19 is an infectious disease caused by the SARS-CoV-2 virus, which rapidly spread worldwide and resulted in a pandemic. Efficient and sensitive detection techniques have been devised since the onset of the epidemic and continue to be improved at present. Due to the crucial role of the SARS-CoV-2 S1 protein in facilitating the virus's entry into cells, efforts in detection and treatment have primarily centered upon this protein.
View Article and Find Full Text PDFDevelopment of transition metal oxide platforms for aptasensing of PSA in cell cultures.
Anal Bioanal Chem
November 2024
Department of Chemistry, Faculty of Science, Ege University, Bornova, 35100, Izmir, Turkey.
In this study, a novel aptasensor based on a transition metal oxide-modified pencil graphite electrode (PGE) was developed for the diagnosis of early-stage prostate cancer (PCa) via monitoring the prostate-specific antigen (PSA), which is the main biomarker for PCa. Single-use PGEs modified with pulsed deposited manganese oxide (MnOx) film were used to attach the amino-terminated aptamer specific to the PSA via carbodiimide chemistry. The designed aptasensor was placed in an electrochemical cell containing ferri/ferrocyanide ions as a redox probe to measure the charge transfer resistances (R) of the electrode surface by electrochemical impedance spectroscopy (EIS) to follow the response of each modification step.
View Article and Find Full Text PDFPolyPyrrole based-impedimetric aptasensor for selective determination of beta-HCG from urine sample.
Bioelectrochemistry
February 2025
Hacettepe University, Department of Chemistry, Ankara, Turkiye. Electronic address:
Herein, a conjugated conducting polymer-based impedimetric aptasensor has been developed to detect beta-human chorionic gonadotropin (bHCG), the one of the important biomarkers in gynecology, from synthetic human urine samples. In this context, gold electrodes were, firstly coated with pyrrole and pyrrole-3-carboxylic acid to obtain the poly(pyrrole-pyrrole-3-carboxylic acid) [poly(Py-PyCOOH)] conductive copolymer by cyclic voltammetry (CV). Then, bHCG-specific peptide aptamer was covalently linked onto the surface via applying a well-known carbodiimide-succinimide chemistry.
View Article and Find Full Text PDFDirect electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of matrix metalloproteinase 2.
Talanta
July 2024
Biosense Institute - Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, Dr Zorana Đinđića 1, 21000 Novi Sad, Serbia. Electronic address:
Simple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction of graphene oxide to produce thin film-modified gold electrodes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!