Highly tunable aptasensing microarrays with graphene oxide multilayers.

Sci Rep

Interdisciplinary School of Green Energy, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.

Published: November 2013

AI Article Synopsis

  • * This array uses fluorescence resonance energy transfer (FRET) to measure protein interactions, where the fluorescence of dye-labeled aptamers is quenched by GO and can be recovered upon protein binding.
  • * The GO films showed impressive quenching ability, detecting human thrombin at very low concentrations, and can be reused multiple times, making it a highly tunable and sustainable sensing platform.

Article Abstract

A highly tunable layer-by-layer (LbL)-assembled graphene oxide (GO) array has been devised for high-throughput multiplex protein sensing. In this array, the fluorescence of different target-bound aptamers labeled with dye is efficiently quenched by GO through fluorescence resonance energy transfer (FRET), and simultaneous multiplex target detection is performed by recovering the quenched fluorescence caused by specific binding between an aptamer and a protein. Thin GO films consisting of 10 bilayers displayed a high quenching ability, yielding over 85% fluorescence quenching with the addition of a 2 μM dye-labeled aptamer. The limit for human thrombin detection in the 6- and 10-bilayered GO array is estimated to be 0.1 and 0.001 nM, respectively, indicating highly tunable nature of LbL assembled GO multilayers in controlling the sensitivity of graphene-based FRET aptasensor. Furthermore, the GO chip could be reused up to four times simply by cleaning it with distilled water.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842537PMC
http://dx.doi.org/10.1038/srep03367DOI Listing

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