Possibilities of application of immunomorphologic methods to gastrointestinal apud cells study were evaluated. These are: immunofluorescent, immunoenzyme, immune-peroxidase, peroxidase-antiperoxidase, avidinbiotin. Causes of immunologic and non-immunologic non-specific background staining and the means of its elimination are discussed, as well as problems of fixation and embedding of the material.
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Article Synopsis
- - The study analyzed changes in the molecular characteristics of podocytes in two kidney conditions: primary focal segmental glomerulosclerosis (pFSGS) and IgA nephropathy (IgAN), using kidney samples from patients and negative controls.
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