Transgene delivery to cultured keratinocytes via replication-deficient adenovirus vectors.

Methods Mol Biol

Graduate Program in Pharmacology & Toxicology, Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, CT, USA.

Published: February 2015

Transient transgene expression can facilitate investigation of that gene-product function or effect on keratinocyte biology. Several chemical and biologic delivery systems are available, and among them adenoviruses offer particular advantages in efficiency and transgene capacity. Here we describe the advantages of bicistronic adenovirus and inclusion of the polycation hexadimethrine bromide to aid in the detection of positively transduced cells and enhance transduction efficiency.

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http://dx.doi.org/10.1007/7651_2013_43DOI Listing

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