AI Article Synopsis
- - The protein BORIS, similar to CTCF, is mainly found in the nucleolus and plays a key role in regulating transcription, though its cytoplasmic functions remain less understood.
- - Research reveals that BORIS possesses a nuclear export signal and interacts with mRNA in neural stem cells and young neurons, showing distinct transcript associations in these cell types.
- - BORIS is linked to actively translating ribosomes, indicating its involvement in gene expression through both transcriptional and post-transcriptional mechanisms.
Article Abstract
Background: BORIS (CTCFL), a paralogue of the multifunctional and ubiquitously expressed transcription factor CTCF, is best known for its role in transcriptional regulation. In the nucleus, BORIS is particularly enriched in the nucleolus, a crucial compartment for ribosomal RNA and RNA metabolism. However, little is known about cytoplasmic BORIS, which represents the major pool of BORIS protein.
Results: We show, firstly, that BORIS has a putative nuclear export signal in the C-terminal domain. Furthermore, BORIS associates with mRNA in both neural stem cells and young neurons. The majority of the BORIS-associated transcripts are different in the two cell types. Finally, by using polysome profiling we show that BORIS is associated with actively translating ribosomes.
Conclusion: We have demonstrated the RNA binding properties of cellular BORIS and its association with actively translating ribosomes. We suggest that BORIS is involved in gene expression at both the transcriptional and post-transcriptional levels.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219345 | PMC |
| http://dx.doi.org/10.1186/1471-2121-14-52 | DOI Listing |
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