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Nonanthocyanin secondary metabolites of black raspberry (Rubus occidentalis L.) fruits: identification by HPLC-DAD, NMR, HPLC-ESI-MS, and ESI-MS/MS analyses. | LitMetric

AI Article Synopsis

  • - The study investigates nonanthocyanin secondary metabolites from black raspberry (Rubus occidentalis L.) to understand their potential role in preventing cell growth (antiproliferative bioactivity).
  • - The metabolites were extracted using ethyl acetate and analyzed through advanced techniques like HPLC and NMR, leading to the identification of several compounds, including quercetin derivatives and various acids.
  • - Remarkably, 15 of these compounds had not been previously documented in black raspberry fruits, expanding the known chemical diversity of this fruit.

Article Abstract

Nonanthocyanin secondary metabolites potentially contributing to the antiproliferative bioactivity of black raspberry ( Rubus occidentalis L.) fruits were extracted in ethyl acetate and isolated by semipreparative and analytical HPLC and analyzed by NMR, HPLC-ESI-MS, and ESI-MS/MS techniques. Here we present complete and partial structures of a variety of the chemical entities such as quercetin 3-glucoside, quercetin 3-rutinoside, myricetin glucoside, dihydrokaempferol glucoside, benzoic acid β-d-glucopyranosyl ester, 3,4-dihydroxybenzoic acid, epicatechin, caffeic acid, p-coumaric acid, p-coumaryl glucoside, p-coumaryl sugar ester, ellagic acid, methyl ellagic acid acetylpentose, methyl ellagic acid valerylpentose, trans-piceid, phloretin glucoside (phloridzin), dihydrosinapic acid, salicylic acid β-d-glucopyranosyl ester, a salicylic acid derivative without attached sugar, p-alkylphenyl glucoside, and a citric acid derivative. To our knowledge, 15 of these compounds were not previously reported in black raspberry fruits.

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Source
http://dx.doi.org/10.1021/jf4039953DOI Listing

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