AI Article Synopsis
- The study examined the use of human umbilical mesenchymal stem cells (HUMSCs) seeded on bladder acellular matrix grafts (BAMG) for bladder reconstruction in dogs.
- HUMSCs were isolated and confirmed positive for specific markers, with bladder defects repaired using either the seeded or unseeded grafts in two groups.
- Results showed that HUMSC-seeded BAMGs led to better regeneration of bladder tissue compared to unseeded grafts, suggesting HUMSCs could be a viable option for bladder tissue engineering.
Article Abstract
Background: The goal of this study was to explore the feasibility of utilizing human umbilical mesenchymal stem cells (HUMSCs)-seeded Bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model.
Methodology/principal Findings: HUMSCs were isolated from newborn umbilical cords and identified by flow cytometry. Partial cystectomy was performed in the experimental and control group. Bladder defects were repaired with HUMSCs-BAMG in the experimental group and repaired with unseeded-BAMG in control group. The implanted grafts were harvested after surgery. H&E and immunohistochemistry staining were performed to evaluate the regeneration of the bladder defect. Primary cultured HUMSCs displayed typical fibroblast morphology with spindle-shaped. Flow cytometry indicated that these cells were positive for CD105 (97.3%) and CD44 (99%), but negative for CD34 (2.8%), CD31 (2.1%), and CD45 (1.7%). Immunohistochemistry staining showed that a multilayered urothelium and well-developed smooth muscle were observed at 12 weeks in experiment group. In contrast, multilayered urothelial tissues were also observed at 12 weeks in group B, but well-developed smooth muscle bundles were observed.
Conclusions/significance: Our preliminary results demonstrate that UMSC-seeded BAMGs are superior to unseeded BAMGs to promote the regeneration of bladder defects. Our findings indicated that HUMSCs may be a potential cell source for bladder tissue engineering.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835736 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0080959 | PLOS |
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