To sustain transgene expression, chitosan was studied to immobilize DNA using layer-by-layer assembly to form polyelectrolyte multilayers (PEMs). Higher DNA concentrations and longer deposition periods demonstrated more DNA adsorptions to PEMs. By adjusting pH and the molecular weight of chitosan, PEM structures were manipulated. Chitosan molecules adsorption to PEMs increased when they were at pH 6 because of their low protonation. Furthermore, the configuration of chitosan favored a coiled-form when the pH was high, as the intramolecular repulsion decreased. Therefore, interdiffusion of polyelectrolytes in PEMs was promoted to increase DNA adsorption, especially for chitosan with high molecular weight. For the release experiments, because PEMs fabricated by lower pH chitosan owned less chitosan molecules, DNA release was enhanced. However, this phenomenon did not happen to chitosan with high molecular weight, which should be due to the entanglement between polymer chains. This comprehensive approach should be beneficial to substrate-mediated gene delivery applications.
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http://dx.doi.org/10.1016/j.carbpol.2013.08.088 | DOI Listing |
Acta Biomater
December 2024
UCD Centre for Biomedical Engineering, University College Dublin, Belfield, Dublin 4, Ireland; School of Mechanical & Materials Engineering, University College Dublin, Belfield, Dublin 4, Ireland; UCD Charles Institute of Dermatology, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland; The Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland. Electronic address:
Microneedle patches (MNs) hold enormous potential to facilitate the minimally-invasive delivery of drugs and vaccines transdermally. However, the micro-mechanics of skin deformation significantly influence the permeation of therapeutics through the skin. Previous studies often fail to appreciate the complexities in microneedle-skin mechanical interactions.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
December 2024
School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China.
A heparinase III (NsHep-III) from Niabella sp. was identified, cloned, and expressed as soluble form in E. coli BL21 (DE3).
View Article and Find Full Text PDFDiabetol Metab Syndr
December 2024
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Obesity is a multifactorial condition influenced by genetic, environmental, and microbiome-related factors. The gut microbiome plays a vital role in maintaining intestinal health, increasing mucus creation, helping the intestinal epithelium mend, and regulating short-chain fatty acid (SCFA) production. These tasks are vital for managing metabolism and maintaining energy balance.
View Article and Find Full Text PDFJ Nanobiotechnology
December 2024
Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
In the post-COVID-19 era, drug-resistant bacterial infections emerge as one of major death causes, where multidrug-resistant Acinetobacter baumannii (MRAB) and drug-resistant Pseudomonas aeruginosa (DRPA) represent primary pathogens. However, the classical antibiotic strategy currently faces the bottleneck of drug resistance. We develop an antimicrobial strategy that applies the selective delivery of CRISPR/Cas9 plasmids to pathogens with biomimetic cationic hybrid vesicles (BCVs), irrelevant to bacterial drug resistance.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
December 2024
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi214023, China.
The present study was aimed to produce the recombinant protein of allergen component 32 (Tyr p 32) and to identify its immunoreactivity. The cDNA encoding Tyr p 32 was amplified from total RNA of and inserted into pET-28a (+) vector. The constructed plasmid pET-28a (+)-Tyr p 32 was transformed into BL21 (DE3) receptor cells.
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