AI Article Synopsis
- The loss of lytic antibodies in Chagas disease patients is an important marker for confirming successful treatment.
- A chemiluminescent enzyme-linked immunosorbent assay was tested for its effectiveness in detecting these antibodies against Trypanosoma cruzi in sera.
- This assay showed high sensitivity (100%) and specificity (98.3%), indicating it can effectively monitor active infections and treatment progress.
Article Abstract
The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970649 | PMC |
| http://dx.doi.org/10.1590/0074-0276130112 | DOI Listing |
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