Eupatorium adenophorum Spreng (E. adenophorum), an invasive plant which has caused widespread poisoning of livestock and crop failures in China. 9-oxo-10, 11-dehydroageraphorone (known as euptox A) is the main toxin causing a series of respiratory and digestive system diseases in animals. The purpose of the research is to isolate and identify the bacteria with the capacity of degrading euptox A, in which way could we lay the foundation in the utilization and prevention for E. adenophorum. Three strains of bacteria with the degradative characteristic are screened from E. adenophorum leaves, soil and goat's rumen respectively by nutrition enrichment. The 16S rDNA sequence of the three isolated strains (XC-07, XC-08, XC-09) were shown to be 97%, 100%, 100% similar to Stenotrophmonas sp., Klebsiella sp. and Pseudomonas sp., respectively, named as Stenotrophomonas sp. XC-07, Klebsiella sp. XC-08 and Pseudomonas sp. XC-09. The degradation rates of 3 strains to euptox A (45 mg/L) in 24 h are 91.2%, 94.3% and 93.2%, respectively, which are determined by HPLC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.toxicon.2013.11.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adenophorone, An Unprecedented Sesquiterpene from Eupatorium adenophorum: Structural Elucidation, Bioinspired Total Synthesis and Neuroprotective Activity Evaluation.
Angew Chem Int Ed Engl
August 2023
Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, P. R. China.
Article Synopsis
- (-)-Adenophorone (1) is a unique tricyclic compound derived from the plant Eupatorium adenopharum, and its structure was confirmed through advanced analytical techniques including X-ray crystallography.* -
- A total synthesis of (-)-Adenophorone was performed, efficiently synthesizing the related compound (+)-euptox A (2) in just eight steps using commercially available materials and demonstrating effective control over molecule orientation.* -
- The synthesized (-)-Adenophorone exhibited significant neuroprotective properties in tested cell lines, supporting its potential therapeutic application and providing insights into its biosynthetic pathway.*
Euptox A Induces G0 /GI arrest and apoptosis of hepatocyte via ROS, mitochondrial dysfunction and caspases-dependent pathways in vivo.
J Toxicol Sci
November 2020
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, China.
As a toxin of Ageratina adenophora (A. adenophora), euptox A (9-oxo-10, 11-dehydroageraphorone) is known to cause hepatotoxicity in animals. In this study, we examined the effects of euptox A on mouse liver cells and its underlying mechanisms for the first time.
View Article and Find Full Text PDFDegradation of euptox A by tannase-producing rumen bacteria from migratory goats.
J Appl Microbiol
November 2017
Regional Station, ICAR-Indian Veterinary Research Institute, Palampur, India.
Aims: The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng).
Methods And Results: Migratory Gaddi goats were selected as source of inoculums for isolating rumen bacteria with ability to produce tannase which catalyses degradation of hydrolysable tannins (HTs).
Euptox A Induces G1 Arrest and Autophagy via p38 MAPK- and PI3K/Akt/mTOR-Mediated Pathways in Mouse Splenocytes.
J Histochem Cytochem
September 2017
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Euptox A (9-oxo-10, 11-dehydroageraphorone), the main toxin isolated from Eupatorium adenophorum, is known to induce immunotoxicity in animals. However, the precise mechanism underlying the effects of Euptox A on splenocytes is unclear. Here, we aimed to investigate the molecular mechanisms underlying the effect of Euptox A in mouse spleens after its intragastric administration and found that Euptox A exhibits proautophagic effects in splenocytes.
View Article and Find Full Text PDFIsolation and identification of bacteria capable of degrading euptox A from Eupatorium adenophorum Spreng.
Toxicon
January 2014
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China. Electronic address:
Eupatorium adenophorum Spreng (E. adenophorum), an invasive plant which has caused widespread poisoning of livestock and crop failures in China. 9-oxo-10, 11-dehydroageraphorone (known as euptox A) is the main toxin causing a series of respiratory and digestive system diseases in animals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!