In this study, the antioxidant properties of Trapa japonica pericarp extracts were evaluated through several biochemical assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH), alkyl radical scavenging activity, hydroxyl radical scavenging, ferric reducing antioxidant power (FRAP) assay, ABTS radical scavenging activity and oxygen radical absorbance capacity (ORAC). The antioxidant activities were compared with other natural and synthetic antioxidants. The results showed that higher radical scavenging activity and antioxidant capacity in FRAP than those of vitamin C as a positive control. T. japonica pericarp extracts have antioxidant properties through its ability to prevent tert-butylhydroperoxide (t-BHP)-induced toxicity which enhance the cell viability, reduce reactive oxygen species (ROS) production, inhibits of oxidative damage and mitochondria dysfunction in Chang liver cells. Therefore, based on these finding, it seems reasonable to suggest that T. japonica pericarp extracts has the potential to protect liver against t-BHP-induced cell damage and should be considered as a potential functional food.
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