AI Article Synopsis
- Raphanus sativus, commonly known as radish, is not only a widely consumed vegetable but also a source of valuable medicinal compounds, though much about its transcriptome remains unknown.
- Approximately 22 million clean reads from leaf tissues were sequenced, leading to the assembly of unigenes, which were annotated using gene ontology and analyzed for key pathways during bolting.
- The study identified 6,295 simple sequence repeats and compared four unigenes with homologs from other plants, contributing to a better understanding of R. sativus's functional genomics and potential therapeutic applications.
Article Abstract
Raphanus sativus is not only a popular edible vegetable but also an important source of medicinal compounds. However, the paucity of knowledge about the transcriptome of R. sativus greatly impedes better understanding of the functional genomics and medicinal potential of R. sativus. In this study, the transcriptome sequencing of leaf tissues in R. sativus was performed for the first time. Approximately 22 million clean reads were generated and used for transcriptome assembly. The generated unigenes were subsequently annotated against gene ontology (GO) database. KEGG analysis further revealed two important pathways in the bolting stage of R.sativus including spliceosome assembly and alkaloid synthesis. In addition, a total of 6,295 simple sequence repeats (SSRs) with various motifs were identified in the unigene library of R. sativus. Finally, four unigenes of R. sativus were selected for alignment with their homologs from other plants, and phylogenetic trees for each of the genes were constructed. Taken together, this study will provide a platform to facilitate gene discovery and advance functional genomic research of R. sativus.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827192 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0080350 | PLOS |
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