AI Article Synopsis
- Polysomal poly(A)RNA from soybean seeds was transcribed by AMV reverse transcriptase and cloned in E. coli using pBR322.
- A bank of mRNA sequences was created from various parts of the soybean plant, such as leaves, axes, and seeds.
- About 13% of the cloned sequences were specific to the maturating stage of the seeds, while over 50% reacted positively with all types of cDNA tested.
Article Abstract
Polysomal poly(A)RNA was transcribed by avian myeloblastosis virus (AMV) reverse transcriptase and double-stranded complementary DNA (cDNA) was then synthesized and cloned in Escherichia coli C600 by (dG)-(dC) tailing in the PstI site of pBR322. A bank of mRNA sequences from maturating soybean seeds (average weight 200 mg) was obtained. (32)P-cDNA based on mRNA from various organs of the plant (leaves, axes, seeds) was synthesized. The clones were hybridized with different kinds of (32)P-cDNA on nitrocellulose filters. Approximately 13% of the clones were specific for the maturating stage, and more than a half the clones gave a positive result with all kinds of cDNA.
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| http://dx.doi.org/10.1007/BF00272636 | DOI Listing |
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