Label-free characterization of biomembranes: from structure to dynamics.

Chem Soc Rev

Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.

Published: February 2014

We review recent progress in the study of the structure and dynamics of phospholipid membranes and associated proteins, using novel label-free analytical tools. We describe these techniques and illustrate them with examples highlighting current capabilities and limitations. Recent advances in applying such techniques to biological and model membranes for biophysical studies and biosensing applications are presented, and future prospects are discussed.

Download full-text PDF

Source
http://dx.doi.org/10.1039/c3cs60243eDOI Listing

Publication Analysis

Top Keywords

structure dynamics
8
label-free characterization
4
characterization biomembranes
4
biomembranes structure
4
dynamics review
4
review progress
4
progress study
4
study structure
4
dynamics phospholipid
4
phospholipid membranes
4

Similar Publications

Flavin adenine nucleotide (FAD)-dependent oxidoreductase enzyme Alcohol oxidase (AOX) facilitates the growth of methylotrophic yeast C. boidinii by catabolizing methanol, producing formaldehyde and hydrogen peroxide. Vacuolar Protease-A (PrA) from C.

View Article and Find Full Text PDF

Journey to the past: molecular de-extinction enables the discovery of ancient β-defensins and highlights their evolutionary history.

Trends Biochem Sci

December 2024

IPSiM, CNRS, INRAE, Institut Agro, Univ. Montpellier, 2, Place P. Viala, F-34 060 Cedex 2 Montpellier, France. Electronic address:

Molecular de-extinction is an innovative science aiming to discover, synthesize, and characterize molecules throughout evolution. Recent work by Ferreira et al. involved mining ancient genomes to search for antimicrobial defensins.

View Article and Find Full Text PDF

Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea.

Dev Biol

December 2024

Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA. Electronic address:

The cell nuclei of Ophisthokonts, the eukaryotic supergroup defined by fungi and metazoans, is remarkable in the constancy of their double-membraned structure in both somatic and germ cells. Such remarkable structural conservation underscores common and ancient evolutionary origins. Yet, the dynamics of disassembly and reassembly displayed by Ophisthokont nuclei vary extensively.

View Article and Find Full Text PDF

Background & Aims: Metabolic dysfunction-associated steatotic liver (MASLD) progression is driven by chronic inflammation and fibrosis, largely influenced by Kupffer cell (KC) dynamics, particularly replenishment of pro-inflammatory monocyte-derived KCs (MoKCs) due to increased death of embryo-derived KCs. Adenosine A3 receptor (A3AR) plays a key role in regulating metabolism and immune responses, making it a promising therapeutic target. This study aimed to investigate the impact of selective A3AR antagonism for regulation of replenished MoKCs, thereby improving MASLD.

View Article and Find Full Text PDF

NSUN2-Mediated R-loop Stabilization as a Key Driver of Bladder Cancer Progression and Cisplatin Sensitivity.

Cancer Lett

December 2024

Department of Urology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, China; Cancer Center, Zhejiang University, Hangzhou, Zhejiang, 310058, China. Electronic address:

R-loops are critical structures that play pivotal roles in regulating genomic stability and modulating gene expression. This study investigates the interactions between the 5-methylcytosine (mC) methyltransferase NOP2/Sun RNA methyltransferase 2 (NSUN2) and R-loops in the transcriptional dynamics and damage repair process of bladder cancer (BCa) cells. We observed markedly elevated levels of R-loops in BCa cells relative to normal urothelial cells.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!