AI Article Synopsis
- Many allergens, like Bet v 1, have similar biophysical traits, particularly the ability to form dimers, but the details of how this occurs are not well understood.
- Researchers discovered that in the structure of dimeric Bet v 1, cysteine replaces the usual tyrosine at position 5, which helps stabilize different dimer configurations through polysulfide bridging.
- These findings highlight residue 5 as a key area linked to allergenic activity in Bet v 1, advancing our knowledge of protein chemistry and the role of modifications in allergy mechanisms.
Article Abstract
Many allergens share several biophysical characteristics, including the capability to undergo oligomerization. The dimerization mechanism in Bet v 1 and its allergenic properties are so far poorly understood. Here, we report crystal structures of dimeric Bet v 1, revealing a noncanonical incorporation of cysteine at position 5 instead of genetically encoded tyrosine. Cysteine polysulfide bridging stabilized different dimeric assemblies, depending on the polysulfide linker length. These dimers represent quaternary arrangements that are frequently observed in related proteins, reflecting their prevalence in unmodified Bet v 1. These conclusions were corroborated by characteristic immunologic properties of monomeric and dimeric allergen variants. Hereby, residue 5 could be identified as an allergenic hot spot in Bet v 1. The presented results refine fundamental principles in protein chemistry and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879576 | PMC |
| http://dx.doi.org/10.1074/jbc.M113.518795 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sustainable Valorization of Rice Straw into Biochar and Carbon Dots Using a Novel One-Pot Approach for Dual Applications in Detection and Removal of Lead Ions.
Nanomaterials (Basel)
January 2025
Faculty of Engineering & Technology, Marwadi University, Rajkot-Morbi Road, Rajkot 360003, Gujarat, India.
Lead (Pb) is a highly toxic heavy metal that causes significant health hazards and environmental damage. Thus, the detection and removal of Pb ions in freshwater sources are imperative for safeguarding public health and the environment. Moreover, the transformation of single resources into multiple high-value products is vital for achieving sustainable development goals (SDGs).
View Article and Find Full Text PDFPerformance, isotherm, kinetics and mechanism of simultaneous removal of Cr(VI), Cu(II) and F ions by CeO-MgO binary oxide nanomaterials.
Sci Rep
January 2025
Department of Physics, Faculty of Science, Islamic University of Madinah, Al-Jamia, Madinah, 42351, Saudi Arabia.
This study focuses on the synthesis of a novel Cerium-Magnesium (CeO-MgO) binary oxide nanomaterials by a simple co-precipitation process and used to remove harmful pollutants such as Cr(VI), Cu(II), and F. The morphology, phase, crystallite size, thermal stability, functional groups, surface area, and porosity of the synthesized nanomaterial were determined by using XRD, SEM, FTIR, TGA/DTA, and BET studies. The prepared nanomaterials showed adsorption selectivity of Cu(II) ≈ F> Cr(VI) with a high adsorption capacity of 84.
View Article and Find Full Text PDFOverview of the epigenetic/cytotoxic dual-target inhibitors for cancer therapy.
Eur J Med Chem
January 2025
School of Medical and Information Engineering, School of Pharmacy, Gannan Medical University, Ganzhou, 341000, PR China; Jiangxi Provincial Key Laboratory of Tissue Engineering, Gannan Medical University, Ganzhou, 341000, PR China. Electronic address:
Epigenetic dysregulation plays a pivotal role in the initiation and progression of various cancers, influencing critical processes such as tumor growth, invasion, migration, survival, apoptosis, and angiogenesis. Consequently, targeting epigenetic pathways has emerged as a promising strategy for anticancer drug discovery in recent years. However, the clinical efficacy of epigenetic inhibitors, such as HDAC inhibitors, has been limited, often accompanied by resistance.
View Article and Find Full Text PDFTriple Combination of MEK, BET, and CDK Inhibitors Significantly Reduces Human Malignant Peripheral Nerve Sheath Tumors in Mouse Models.
Clin Cancer Res
December 2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Barcelona, Spain.
Purpose: Malignant peripheral nerve sheath tumor (MPNST) is an aggressive soft tissue sarcoma that develops sporadically or in Neurofibromatosis type 1 patients. Its development is marked by the inactivation of specific tumor suppressor genes (TSGs): NF1, CDKN2A and SUZ12EED (Polycomb Repressor Complex 2). Each TSG loss can be targeted by particular drug inhibitors and we aimed to systematically combine these inhibitors, guided by TSG inactivation status, to test their precision medicine potential for MPNSTs.
View Article and Find Full Text PDFHighly porous activated carbon derived from the papaya plant (stems and leaves) for superior adsorption of alizarin red s and methylene blue dyes from wastewater.
RSC Adv
January 2025
Chemistry Department, Faculty of Science, Mansoura University Mansoura 35516 Egypt +201000166374.
In this study, stems and leaves of the papaya plant were employed to prepare a high-quality porous adsorbent carbonization and chemical activation using phosphoric acid. This adsorbent demonstrates superior adsorption capabilities for the efficient removal of hazardous alizarin red s (ARS) and methylene blue (MB) dyes. Thus, it contributes to waste reduction and promotes sustainable practices in environmental remediation, aligning with global efforts to develop sustainable materials that address water pollution while supporting circular economy principles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!